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US 12,761,209 B2Granted
Devices and methods to induce adult type maturation of human pluripotent stem cell derived cardiomyocytes
AssigneesUniversity of Connecticut, The Regents of the University of California
InventorsKshitiz, Junaid Afzal, Wenqiang Du, Yasir Suhail
Life of this patent
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Abstract
Disclosed are constructs and methods to accelerate maturation of human pluripotent stem cell derived cardiomyocytes by maintaining them on a Cardiac Mimetic Matrix (CMM) substrate.
Claims16
A construct, comprising:
- (a) a patterned scaffold, wherein the patterned scaffold comprises a polymeric hydrogel substrate comprising a plurality of nanowrinkles, wherein the nanowrinkles comprise branched folds directionally aligned over a centimeter length scale, wherein the polymeric substrate has a viscoelasticity between about 15 kPa and about 100kPa;
- (b) one or more cardiac matrix ligands conjugated to the patterned scaffold, wherein the one or more cardiac matrix ligands comprises 1, 2, 3, 4 or more of nephronectin (SEQ ID NO:
- 13), GRGDS (SEQ ID NO:
- 10), GFOGER (SEQ ID NO:
- 11), GFPGER (SEQ ID NO:
- 12) and/or other peptides containing one or more RGD motifs.
3 more elements
Dependencies are inferred from the claim text.