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AssigneeTianjin Yeahe Biotechnology Co., Ltd.
InventorsYanhe Ma, Ting Shi, Yunjie Li, Pingping Han, Yuan Li
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Provided are a Bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.
A Bacillus subtilis genetically engineered bacterium for producing tagatose, characterized in that said genetically engineered bacterium is a Bacillus subtilis genetically engineered bacterium co-expressing an α-glucan phosphorylase gene from Thermococcus kodakarensis, a glucose phosphomutase gene from Thermococcus kodakarensis, a glucose phosphate isomerase gene from Thermus thermophilus, a 6-tagatose phosphate epimerase gene from Thermoanaerobacter indiensis, and a 6-tagatose phosphate phosphatase gene from Archaeoglobus fulgidus, or a mixture of Bacillus subtilis genetically engineered bacteria respectively expressing an α-glucan phosphorylase gene from Thermococcus kodakarensis, a glucose phosphomutase gene from Thermococcus kodakarensis, a glucose phosphate isomerase gene from Thermus thermophilus, a 6-tagatose phosphate epimerase gene from Thermoanaerobacter indiensis, and a 6-tagatose phosphate phosphatase gene from Archaeoglobus fulgidus.
An expression vector, characterized in that it comprises an α-glucan phosphorylase gene from Thermococcus kodakarensis, a glucose phosphomutase gene from Thermococcus kodakarensis, a glucose phosphate isomerase gene from Thermus thermophilus, a 6-tagatose phosphate epimerase gene from Thermoanaerobacter indiensis, and a 6-tagatose phosphate phosphatase gene from Archaeoglobus fulgidus and is capable of co-expressing these genes.
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