US 12,760,340 B2Granted

Methods and compositions for the production of rhamnolipid

AssigneeThe Regents of the University of California

InventorsDeepika Awasthi, Steven W. Singer

Life of this patent

Estimating the term…

Abstract

The present invention provides for a method for producing a rhamnolipid, the method comprising: (a) providing a genetically modified host cell comprising one or more of RhlYZAB capable of expression in the host cell in a growth or culture medium; (b) growing or culturing the host cell such that the one or more of RhlYZAB are expressed and a rhamnolipid is produced; and (c) optionally recovering the rhamnolipid from the host cell or from the growth or culture medium. In some embodiments, the host cell is a methanotroph.

Claims9

Dependent claims

A genetically modified methanotroph comprising one or more of enoyl-CoA hydratase/isomerase (RhlY and RhlZ), 3-hydroxyacyl-ACP-O-3 hydroxyacyltransferase (RhlA), or rhamnosyl transferase (RhlB), and one or more of the following mutations:

#CHROM
POS
REF
ALT
NC 016112.1
257711
T
C
NC 016112.1
274084
A
C
NC 016112.1
274102
A
G
NC 016112.1
274387
C
T
NC 016112.1
274678
T
C
NC 016112.1
274869
C
T
NC 016112.1
275047
T
C
NC 016112.1
275049
G
A
NC 016112.1
275058
C
T
NC 016112.1
275059
G
T
NC 016112.1
275081
C
T
NC 016112.1
275093
C
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NC 016112.1
275110
T
C
NC 016112.1
278205
C
G
NC 016112.1
278215
A
G
NC 016112.1
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T
G
NC 016112.1
278240
G
A
NC 016112.1
278284
G
A
NC 016112.1
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G
A
NC 016112.1
278466
G
T
NC 016112.1
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A
G
NC 016112.1
278492
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C
NC 016112.1
278494
G
T
NC 016112.1
278514
T
A
NC 016112.1
278515
C
G
NC 016112.1
278517
C
T
NC 016112.1
278521
G
A
NC 016112.1
278545
A
G
NC 016112.1
279003
A
G
NC 016112.1
279129
C
T
NC 016112.1
279280
G
T
NC 016112.1
279714
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NC 016112.1
279721
T
C
NC 016112.1
279732
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G
NC 016112.1
280003
A
C
NC 016112.1
280038
T
C
NC 016112.1
280048
A
G
NC 016112.1
280050
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C
NC 016112.1
280053
C
T
NC 016112.1
280077
C
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NC 016112.1
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A
NC 016112.1
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NC 016112.1
280275
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NC 016112.1
280293
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NC 016112.1
280317
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A
NC 016112.1
280322
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NC 016112.1
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NC 016112.1
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280402
T
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NC 016112.1
280495
T
C
NC 016112.1
280585
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NC 016112.1
280588
A
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NC 016112.1
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A
G
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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A
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NC 016112.1
283164
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283170
A
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NC 016112.1
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NC 016112.1
283185
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
284046
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A
NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
284399
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NC 016112.1
284404
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NC 016112.1
284407
G
A
NC 016112.1
284534
A
C
NC 016112.1
284560
C
A
NC 016112.1
284571
G
C
NC 016112.1
284605
G
T
NC 016112.1
284710
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NC 016112.1
284748
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NC 016112.1
284829
A
G
NC 016112.1
284833
A
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NC 016112.1
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A
NC 016112.1
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NC 016112.1
284866
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NC 016112.1
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NC 016112.1
284870
A
C
NC 016112.1
284872
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A
NC 016112.1
284909
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NC 016112.1
284912
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A
NC 016112.1
284924
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NC 016112.1
284926
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NC 016112.1
284935
G
A
NC 016112.1
284943
G
A
NC 016112.1
284983
A
C
NC 016112.1
284987
C
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NC 016112.1
284989
A
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NC 016112.1
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NC 016112.1
285006
A
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NC 016112.1
285007
A
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NC 016112.1
285010
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NC 016112.1
285011
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NC 016112.1
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T
G
NC 016112.1
285020
A
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NC 016112.1
285023
A
G
NC 016112.1
285033
G
A
NC 016112.1
285053
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A
NC 016112.1
285058
G
C
NC 016112.1
285062
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NC 016112.1
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NC 016112.1
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NC 016112.1
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A
NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
285153
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
504062
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
504109
T
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NC 016112.1
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A
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NC 016112.1
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C
T
NC 016112.1
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G
C
NC 016112.1
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NC 016112.1
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A
NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
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NC 016112.1
2028111
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2637153
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NC 016112.1
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T
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A
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NC 016112.1
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NC 016112.1
4250113
C
T
NC 016112.1
4250115
T
C
NC 016112.1
4250119
T
A
NC 016112.1
4250142
A
G
NC 016112.1
4250148
T
C
NC 016112.1
4250154
G
A
NC 016112.1
4250157
G
A
NC 016112.1
4250166
C
T
NC 016112.1
4250169
C
T
NC 016112.1
4250172
G
A
NC 016112.1
4250199
T
C
NC 016112.1
4250200
T
G
NC 016112.1
4250205
T
C
NC 016112.1
4250235
G
A
NC 016112.1
4250253
T
G
NC 016112.1
4250319
G
A
NC 016112.1
4250546
G
A
NC 016112.1
4250584
T
C
NC 016112.1
4250678
C
T
NC 016112.1
4250712
A
T
NC 016112.1
4250808
A
G
NC 016112.1
4250821
C
T
NC 016112.1
4250837
G
C
NC 016112.1
4250863
T
A
NC 016112.1
4250879
A
G
NC 016112.1
4250924
A
G
NC 016112.1
4255137
C
T
NC 016112.1
4255200
C
T
NC 016112.1
4258918
T
A
NC 016112.1
4258932
A
G
NC 016112.1
4258938
G
A
NC 016112.1
4258963
A
T
NC 016112.1
4258972
C
T
NC 016112.1
4259013
A
G
NC 016112.1
4259401
C
T
NC 016112.1
4259421
T
C
NC 016112.1
4259727
T
C
NC 016112.1
4262921
A
G
NC 016112.1
4262948
A
G
NC 016112.1
4263098
C
T
NC 016112.1
4263149
G
T
NC 016112.1
4263405
T
G
NC 016112.1
4263776
T
A
NC 016112.1
4263947
C
G
NC 016112.1
4263958
T
C
NC 016112.1
4263968
C
T
NC 016112.1
4263979
A
G
NC 016112.1
4270962
C
T
NC 016112.1
4270965
A
G
NC 016112.1
4270968
T
C
NC 016112.1
4271667
C
A
NC 016112.1
4271679
C
G
NC 016112.1
4271701
T
A
NC 016112.1
4428308
G
C
NC 016112.1
4552963
T
C
NC 016112.1
4553104
G
A
NC 016112.1
4553506
C
T
NC 016112.1
4553743
A
G
NC 016112.1
4554354
T
C
NC 016108.1
128058
G
C;
wherein the genetically modified methanotroph is a Methylotuvimicrobium cell.

1,918 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/324,283Filed 26 May 2023 · us provisional application
Priority: 27 May 2022
Published: US 2023/0399666 A114 Dec 2023
Granted: 13 Oct 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12P 7/6409
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group;…:…
C12N 9/1029
Acyltransferases: transferring groups other than amino-acyl groups
C12N 9/1051
Glycosyltransferases: hexosyltransferases
C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases

Family

Application63/346,788Us provisional application · filed 27 May 2022
US 2023/0399666 A1Pre-grant publication · published 14 Dec 2023
US 12,760,340 B2This patent

From the US filing history. Foreign family members aren’t listed.

Timeline

Citations

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Abstract

#CHROM
POS
REF
ALT
NC 016112.1
257711
T
C
NC 016112.1
274084
A
C
NC 016112.1
274102
A
G
NC 016112.1
274387
C
T
NC 016112.1
274678
T
C
NC 016112.1
274869
C
T
NC 016112.1
275047
T
C
NC 016112.1
275049
G
A
NC 016112.1
275058
C
T
NC 016112.1
275059
G
T
NC 016112.1
275081
C
T
NC 016112.1
275093
C
T
NC 016112.1
275110
T
C
NC 016112.1
278205
C
G
NC 016112.1
278215
A
G
NC 016112.1
278222
T
G
NC 016112.1
278240
G
A
NC 016112.1
278284
G
A
NC 016112.1
278386
G
A
NC 016112.1
278466
G
T
NC 016112.1
278490
A
G
NC 016112.1
278492
T
C
NC 016112.1
278494
G
T
NC 016112.1
278514
T
A
NC 016112.1
278515
C
G
NC 016112.1
278517
C
T
NC 016112.1
278521
G
A
NC 016112.1
278545
A
G
NC 016112.1
279003
A
G
NC 016112.1
279129
C
T
NC 016112.1
279280
G
T
NC 016112.1
279714
T
C
NC 016112.1
279721
T
C
NC 016112.1
279732
C
G
NC 016112.1
280003
A
C
NC 016112.1
280038
T
C
NC 016112.1
280048
A
G
NC 016112.1
280050
T
C
NC 016112.1
280053
C
T
NC 016112.1
280077
C
T
NC 016112.1
280080
G
A
NC 016112.1
280256
G
A
NC 016112.1
280275
T
C
NC 016112.1
280293
T
C
NC 016112.1
280317
C
A
NC 016112.1
280322
A
G
NC 016112.1
280378
T
C
NC 016112.1
280393
C
G
NC 016112.1
280402
T
C
NC 016112.1
280495
T
C
NC 016112.1
280585
T
C
NC 016112.1
280588
A
C
NC 016112.1
280630
A
G
NC 016112.1
280681
C
A
NC 016112.1
280705
C
T
NC 016112.1
280708
T
C
NC 016112.1
280711
C
T
NC 016112.1
280714
G
T
NC 016112.1
280717
T
C
NC 016112.1
280729
T
C
NC 016112.1
280731
T
C
NC 016112.1
280754
C
T
NC 016112.1
280825
T
G
NC 016112.1
280885
C
T
NC 016112.1
280913
A
G
NC 016112.1
280914
T
C
NC 016112.1
280936
C
A
NC 016112.1
280957
T
C
NC 016112.1
281142
A
G
NC 016112.1
281323
G
A
NC 016112.1
281338
A
G
NC 016112.1
281353
A
G
NC 016112.1
281461
T
A
NC 016112.1
281638
C
T
NC 016112.1
282166
C
A
NC 016112.1
282168
G
A
NC 016112.1
282169
C
T
NC 016112.1
282235
A
T
NC 016112.1
282262
A
T
NC 016112.1
282400
C
T
NC 016112.1
282479
T
C
NC 016112.1
282486
C
T
NC 016112.1
282727
C
T
NC 016112.1
282835
T
C
NC 016112.1
282955
C
T
NC 016112.1
282965
T
C
NC 016112.1
282967
G
A
NC 016112.1
283006
A
C
NC 016112.1
283043
A
T
NC 016112.1
283077
G
T
NC 016112.1
283164
G
A
NC 016112.1
283170
A
G
NC 016112.1
283173
G
C
NC 016112.1
283185
G
A
NC 016112.1
283216
T
C
NC 016112.1
283236
A
T
NC 016112.1
283248
C
T
NC 016112.1
283275
G
A
NC 016112.1
283278
C
T
NC 016112.1
283336
C
T
NC 016112.1
283365
G
C
NC 016112.1
283389
G
A
NC 016112.1
283395
C
T
NC 016112.1
283440
T
C
NC 016112.1
283458
T
C
NC 016112.1
283461
T
C
NC 016112.1
283503
C
T
NC 016112.1
283533
A
C
NC 016112.1
283572
G
A
NC 016112.1
283578
C
T
NC 016112.1
283629
T
C
NC 016112.1
283641
G
T
NC 016112.1
283647
C
T
NC 016112.1
283792
A
G
NC 016112.1
283869
A
G
NC 016112.1
283893
T
C
NC 016112.1
283920
A
C
NC 016112.1
283934
G
A
NC 016112.1
283953
A
G
NC 016112.1
283974
T
C
NC 016112.1
284034
T
C
NC 016112.1
284040
C
T
NC 016112.1
284046
G
A
NC 016112.1
284103
A
G
NC 016112.1
284107
G
A
NC 016112.1
284273
T
C
NC 016112.1
284392
C
G
NC 016112.1
284394
G
A
NC 016112.1
284395
G
C
NC 016112.1
284399
G
A
NC 016112.1
284404
C
T
NC 016112.1
284407
G
A
NC 016112.1
284534
A
C
NC 016112.1
284560
C
A
NC 016112.1
284571
G
C
NC 016112.1
284605
G
T
NC 016112.1
284710
C
T
NC 016112.1
284748
C
T
NC 016112.1
284829
A
G
NC 016112.1
284833
A
G
NC 016112.1
284857
G
A
NC 016112.1
284860
C
T
NC 016112.1
284866
C
T
NC 016112.1
284869
A
G
NC 016112.1
284870
A
C
NC 016112.1
284872
G
A
NC 016112.1
284909
G
A
NC 016112.1
284912
G
A
NC 016112.1
284924
C
T
NC 016112.1
284926
G
T
NC 016112.1
284935
G
A
NC 016112.1
284943
G
A
NC 016112.1
284983
A
C
NC 016112.1
284987
C
T
NC 016112.1
284989
A
G
NC 016112.1
285002
C
T
NC 016112.1
285006
A
G
NC 016112.1
285007
A
C
NC 016112.1
285010
T
G
NC 016112.1
285011
C
T
NC 016112.1
285019
T
G
NC 016112.1
285020
A
G
NC 016112.1
285023
A
G
NC 016112.1
285033
G
A
NC 016112.1
285053
T
A
NC 016112.1
285058
G
C
NC 016112.1
285062
C
T
NC 016112.1
285068
T
G
NC 016112.1
285069
C
T
NC 016112.1
285076
G
A
NC 016112.1
285088
T
C
NC 016112.1
285091
A
G
NC 016112.1
285092
C
T
NC 016112.1
285095
A
G
NC 016112.1
285100
T
A
NC 016112.1
285109
G
T
NC 016112.1
285114
T
C
NC 016112.1
285115
T
C
NC 016112.1
285118
A
G
NC 016112.1
285153
G
A
NC 016112.1
503932
A
G
NC 016112.1
503936
T
C
NC 016112.1
503940
G
A
NC 016112.1
503945
A
G
NC 016112.1
503949
A
C
NC 016112.1
503970
C
T
NC 016112.1
503972
G
A
NC 016112.1
503979
A
G
NC 016112.1
504062
C
A
NC 016112.1
504071
A
G
NC 016112.1
504073
T
A
NC 016112.1
504091
C
G
NC 016112.1
504109
T
C
NC 016112.1
504856
A
T
NC 016112.1
504907
C
T
NC 016112.1
538607
G
C
NC 016112.1
705301
C
G
NC 016112.1
790204
G
A
NC 016112.1
1075721
C
A
NC 016112.1
1116663
C
G
NC 016112.1
1116664
G
C
NC 016112.1
1330008
G
T
NC 016112.1
1330095
A
G
NC 016112.1
1393728
C
T
NC 016112.1
1419041
T
C
NC 016112.1
1439362
C
A
NC 016112.1
1479855
T
C
NC 016112.1
1480356
C
T
NC 016112.1
1593190
T
A
NC 016112.1
1593227
A
G
NC 016112.1
1593247
G
A
NC 016112.1
1593316
G
A
NC 016112.1
1593337
T
C
NC 016112.1
1593340
G
A
NC 016112.1
1593353
T
C
NC 016112.1
1593360
C
T
NC 016112.1
1593364
C
T
NC 016112.1
1593444
T
G
NC 016112.1
1593445
T
G
NC 016112.1
1593520
G
A
NC 016112.1
1593525
G
A
NC 016112.1
1593540
T
G
NC 016112.1
1593565
G
A
NC 016112.1
1593577
T
C
NC 016112.1
1593583
T
C
NC 016112.1
1593586
C
T
NC 016112.1
1593589
C
G
NC 016112.1
1593595
G
A
NC 016112.1
1593598
A
G
NC 016112.1
1593612
T
C
NC 016112.1
1593613
G
A
NC 016112.1
1593633
A
G
NC 016112.1
1593634
T
C
NC 016112.1
1593639
A
T
NC 016112.1
1593641
A
G
NC 016112.1
1593644
C
A
NC 016112.1
1593645
G
A
NC 016112.1
1593652
C
A
NC 016112.1
1593669
G
A
NC 016112.1
1593683
C
T
NC 016112.1
1594017
A
G
NC 016112.1
1594020
G
A
NC 016112.1
1594023
A
G
NC 016112.1
1594053
G
T
NC 016112.1
1594054
T
G
NC 016112.1
1594125
C
T
NC 016112.1
1594206
C
T
NC 016112.1
1594314
C
T
NC 016112.1
1594377
C
A
NC 016112.1
1594425
C
T
NC 016112.1
1594428
C
A
NC 016112.1
1594434
G
T
NC 016112.1
1594479
T
G
NC 016112.1
1594518
C
T
NC 016112.1
1594520
T
G
NC 016112.1
1594524
A
G
NC 016112.1
1594549
T
C
NC 016112.1
1594575
C
T
NC 016112.1
1594578
G
A
NC 016112.1
1594602
T
C
NC 016112.1
1594612
C
G
NC 016112.1
1594656
C
G
NC 016112.1
1594662
C
T
NC 016112.1
1594683
A
T
NC 016112.1
1594701
G
C
NC 016112.1
1594704
A
C
NC 016112.1
1594710
T
C
NC 016112.1
1594713
C
T
NC 016112.1
1594728
A
G
NC 016112.1
1594732
G
A
NC 016112.1
1594734
G
C
NC 016112.1
1594769
T
C
NC 016112.1
1594793
A
G
NC 016112.1
1594809
G
A
NC 016112.1
1594815
G
A
NC 016112.1
1594819
C
T
NC 016112.1
1594821
G
C
NC 016112.1
1594830
T
A
NC 016112.1
1594863
C
T
NC 016112.1
1594870
G
A
NC 016112.1
1595111
G
A
NC 016112.1
1595177
G
A
NC 016112.1
1595180
A
G
NC 016112.1
1595273
T
C
NC 016112.1
1595315
A
C
NC 016112.1
1595342
A
C
NC 016112.1
1595354
G
A
NC 016112.1
1595360
T
C
NC 016112.1
1595384
A
G
NC 016112.1
1595426
C
T
NC 016112.1
1595429
G
A
NC 016112.1
1595432
C
T
NC 016112.1
1595435
A
G
NC 016112.1
1595453
G
A
NC 016112.1
1595454
C
A
NC 016112.1
1595504
T
A
NC 016112.1
1595546
G
A
NC 016112.1
1595567
G
A
NC 016112.1
1595627
C
T
NC 016112.1
1595651
C
T
NC 016112.1
1598357
T
C
NC 016112.1
1598396
G
A
NC 016112.1
1598399
A
G
NC 016112.1
1598434
G
T
NC 016112.1
1598435
G
A
NC 016112.1
1598477
A
G
NC 016112.1
1598486
A
G
NC 016112.1
1598507
C
T
NC 016112.1
1598528
C
T
NC 016112.1
1598531
T
C
NC 016112.1
1598567
G
A
NC 016112.1
1598579
G
C
NC 016112.1
1598582
C
A
NC 016112.1
1598606
T
C
NC 016112.1
1598618
G
T
NC 016112.1
1598627
G
A
NC 016112.1
1598639
A
G
NC 016112.1
1598642
G
A
NC 016112.1
1598645
G
A
NC 016112.1
1598657
G
A
NC 016112.1
1598689
A
G
NC 016112.1
1598696
A
C
NC 016112.1
1598702
G
A
NC 016112.1
1598717
C
T
NC 016112.1
1598780
T
C
NC 016112.1
1598789
A
G
NC 016112.1
1598852
A
G
NC 016112.1
1598870
A
T
NC 016112.1
1598939
G
C
NC 016112.1
1599023
G
T
NC 016112.1
1599026
T
C
NC 016112.1
1599035
G
A
NC 016112.1
1599038
C
G
NC 016112.1
1599053
G
A
NC 016112.1
1599104
C
T
NC 016112.1
1599140
A
G
NC 016112.1
1599181
T
C
NC 016112.1
1599182
T
G
NC 016112.1
1599201
A
G
NC 016112.1
1599206
C
T
NC 016112.1
1599210
G
C
NC 016112.1
1599216
A
C
NC 016112.1
1599217
T
C
NC 016112.1
1599227
A
T
NC 016112.1
1815912
G
C
NC 016112.1
2028111
C
T
NC 016112.1
2637141
T
C
NC 016112.1
2637145
C
T
NC 016112.1
2637153
G
A
NC 016112.1
2674948
A
G
NC 016112.1
2674956
A
G
NC 016112.1
2674965
T
G
NC 016112.1
2674967
A
G
NC 016112.1
2674973
T
C
NC 016112.1
2675002
A
G
NC 016112.1
2675003
T
G
NC 016112.1
2675021
A
G
NC 016112.1
2675077
A
G
NC 016112.1
2675094
G
A
NC 016112.1
2703406
C
G
NC 016112.1
2703409
A
G
NC 016112.1
3190412
T
C
NC 016112.1
3190432
G
A
NC 016112.1
3190464
T
C
NC 016112.1
3797919
C
T
NC 016112.1
3798246
T
C
NC 016112.1
3798385
A
G
NC 016112.1
3798455
C
G
NC 016112.1
3798478
A
C
NC 016112.1
3798479
T
C
NC 016112.1
3798503
T
C
NC 016112.1
4222340
T
G
NC 016112.1
4222349
A
C
NC 016112.1
4233436
G
T
NC 016112.1
4233466
G
A
NC 016112.1
4233475
A
G
NC 016112.1
4233780
C
T
NC 016112.1
4233854
T
C
NC 016112.1
4239364
A
G
NC 016112.1
4239375
T
G
NC 016112.1
4239378
A
G
NC 016112.1
4249077
T
C
NC 016112.1
4249101
A
T
NC 016112.1
4249149
T
C
NC 016112.1
4249152
A
G
NC 016112.1
4249185
A
G
NC 016112.1
4249212
C
T
NC 016112.1
4249287
A
T
NC 016112.1
4249322
C
T
NC 016112.1
4249335
A
G
NC 016112.1
4249392
G
A
NC 016112.1
4249608
T
C
NC 016112.1
4249620
G
T
NC 016112.1
4249623
T
C
NC 016112.1
4249634
A
G
NC 016112.1
4249635
A
G
NC 016112.1
4249670
A
G
NC 016112.1
4249692
C
T
NC 016112.1
4249695
C
T
NC 016112.1
4249717
C
T
NC 016112.1
4249809
T
C
NC 016112.1
4249824
G
A
NC 016112.1
4249842
T
C
NC 016112.1
4249845
C
G
NC 016112.1
4249860
T
C
NC 016112.1
4249874
T
C
NC 016112.1
4249883
A
G
NC 016112.1
4249893
C
T
NC 016112.1
4249902
T
C
NC 016112.1
4249908
A
G
NC 016112.1
4249911
A
G
NC 016112.1
4249912
A
C
NC 016112.1
4249914
C
T
NC 016112.1
4249917
A
G
NC 016112.1
4249926
T
C
NC 016112.1
4249986
A
G
NC 016112.1
4250010
T
C
NC 016112.1
4250103
G
A
NC 016112.1
4250110
A
C
NC 016112.1
4250113
C
T
NC 016112.1
4250115
T
C
NC 016112.1
4250119
T
A
NC 016112.1
4250142
A
G
NC 016112.1
4250148
T
C
NC 016112.1
4250154
G
A
NC 016112.1
4250157
G
A
NC 016112.1
4250166
C
T
NC 016112.1
4250169
C
T
NC 016112.1
4250172
G
A
NC 016112.1
4250199
T
C
NC 016112.1
4250200
T
G
NC 016112.1
4250205
T
C
NC 016112.1
4250235
G
A
NC 016112.1
4250253
T
G
NC 016112.1
4250319
G
A
NC 016112.1
4250546
G
A
NC 016112.1
4250584
T
C
NC 016112.1
4250678
C
T
NC 016112.1
4250712
A
T
NC 016112.1
4250808
A
G
NC 016112.1
4250821
C
T
NC 016112.1
4250837
G
C
NC 016112.1
4250863
T
A
NC 016112.1
4250879
A
G
NC 016112.1
4250924
A
G
NC 016112.1
4255137
C
T
NC 016112.1
4255200
C
T
NC 016112.1
4258918
T
A
NC 016112.1
4258932
A
G
NC 016112.1
4258938
G
A
NC 016112.1
4258963
A
T
NC 016112.1
4258972
C
T
NC 016112.1
4259013
A
G
NC 016112.1
4259401
C
T
NC 016112.1
4259421
T
C
NC 016112.1
4259727
T
C
NC 016112.1
4262921
A
G
NC 016112.1
4262948
A
G
NC 016112.1
4263098
C
T
NC 016112.1
4263149
G
T
NC 016112.1
4263405
T
G
NC 016112.1
4263776
T
A
NC 016112.1
4263947
C
G
NC 016112.1
4263958
T
C
NC 016112.1
4263968
C
T
NC 016112.1
4263979
A
G
NC 016112.1
4270962
C
T
NC 016112.1
4270965
A
G
NC 016112.1
4270968
T
C
NC 016112.1
4271667
C
A
NC 016112.1
4271679
C
G
NC 016112.1
4271701
T
A
NC 016112.1
4428308
G
C
NC 016112.1
4552963
T
C
NC 016112.1
4553104
G
A
NC 016112.1
4553506
C
T
NC 016112.1
4553743
A
G
NC 016112.1
4554354
T
C
NC 016108.1
128058
G
C;
wherein the genetically modified methanotroph is a Methylotuvimicrobium cell.

1,918 more elements

Facts

Application

18/324,283Filed 26 May 2023 · us provisional application

Priority

27 May 2022

Published

US 2023/0399666 A114 Dec 2023

Granted

13 Oct 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12P 7/6409
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group;…:…
C12N 9/1029
Acyltransferases: transferring groups other than amino-acyl groups
C12N 9/1051
Glycosyltransferases: hexosyltransferases
C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases