Production of odd chain fatty acid derivatives in recombinant microbial cells

A recombinant microbial cell comprising:

• (a) polynucleotides encoding polypeptides having enzymatic activity effective to produce an increased amount of propionyl-CoA in the recombinant microbial cell relative to the amount of propionyl-CoA produced in a parental microbial cell lacking or having a reduced amount of said enzymatic activity, wherein the polynucleotides encode
• (i) one or more of a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 40, 41, 42, or 43, wherein the polypeptide has (R)-citramalate synthase activity;
• a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 44 or 46 and 45 or 47, wherein the polypeptide has isopropylmalate isomerase activity;
• and a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 48, 49, or 50, wherein the polypeptide has beta-isopropylmalate dehydrogenase activity, and/or
• (ii) one or more of a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 20, 21, 22, 23, or 24, wherein the polypeptide has aspartokinase activity;
• a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 20, 21, 25, 26, or 27, wherein the polypeptide has homoserine dehydrogenase activity;
• a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 28, 29, 30, or 31, wherein the polypeptide has homoserine kinase activity;
• a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 32, 33, or 34, wherein the polypeptide has threonine synthase activity;
• and a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 35, 36, 37, 38, or 39, wherein the polypeptide has threonine deaminase activity,
• (b) a polynucleotide encoding a polypeptide having β-ketoacyl-ACP synthase activity that utilizes propionyl-CoA as a substrate, wherein the polypeptide having β-ketoacyl-ACP synthase activity comprises one or more sequence motif selected from SEQ ID NO:
• 14, 15, 16, 17, 18, or 19, and
• (c) a polynucleotide encoding a polypeptide comprising at least 85% sequence identity to SEQ ID NOs:
• 64, 65, 66, 67, 68, 69, 70, 71, or 72, wherein the polypeptide has thioesterase enzyme activity,
• wherein the recombinant microbial cell produces a fatty acid derivative composition comprising odd chain and even chain fatty acid derivatives when cultured in the presence of a carbon source under conditions effective to express the polynucleotides according to (a), (b), and (c), and
• wherein at least 10% of the fatty acid derivatives in the fatty acid derivative composition are odd chain fatty acid derivatives.