CAS12 proteins and uses thereof
AssigneesReforgene Medicine, Zhejiang Synsorbio Technology Co., Ltd.
InventorsJunbin Liang, Liancheng Huang, Chongjian Chen, Yang Sun, Weiye Pan, Hui Xu, Kaiwei Si, Jinxiu Cai, Qing Liao, Desheng Huangfu
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Abstract
Cas12 protein, guide polynucleotide, inactivated Cas12 mutant, fusion protein or conjugate including the Cas12 protein, isolated nucleic acid, CRISPR-Cas12 system, vector system, delivery system, cell, pharmaceutical composition, and kit, and the use thereof are provided.
Claims10
A non-naturally occurring Cas12 protein, wherein the Cas12 protein comprises an amino acid sequence having at least 70% sequence identity to the amino acid sequence shown in SEQ ID NO:
- 696, wherein the Cas12 protein has one or more mutations at amino acid residues with corresponding positions in the sequence shown in SEQ ID NO:
- 696;
- the Cas12 protein forms a complex with a guide polynucleotide, the guide polynucleotide comprises a guide sequence that is reverse complementary to a target nucleic acid, and
- the guide polynucleotide comprises a scaffold sequence that interacts with the Cas12 protein.
A fusion protein or conjugate, comprising:
- (1) a Cas12 protein, wherein the Cas12 protein comprises an amino acid sequence having at least 70% sequence identity to the amino acid sequence shown in SEQ ID NO:
- 696;
- and
- (2) a homologous or heterologous functional domain.
Dependencies are inferred from the claim text.