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US 12,760,316 B2Granted
IgG variants for induction of immune response without adjuvant
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsHugh Mason, Andrew Diamos, Mary Pardhe
Life of this patent
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Abstract
The disclosure relates to immunoglobulin variants that are useful for the design of vaccinations against a variety of pathogens and their method of production and use. In some aspects, the immunoglobulin variants are variants of IgG, for example, the 6D8 antibody.
Claims5
A recombinant protein comprising:
- a unit of a Fc fusion comprising a CH2 domain and a CH3 domain of an IgG;
- a unit of an antigen, wherein the unit of the antigen is not an epitope of the IgG;
- a unit of a variable heavy chain (VH) domain of the IgG;
- and
- a unit of a CH1 domain of the IgG;
- an epitope tag consisting of the sequence YKLDIS (SEQ ID NO. 1);
- a unit of a light chain variable (VL) domain of the IgG;
- and
- a unit of a light chain constant (CL) domain of the IgG,
- wherein:
- the unit of the antigen is linked to the unit of the VH domain of the IgG at the N-terminus or to the CH3 domain of IgG at the C-terminus;
- the unit of the CH1 domain of the IgG is linked to the CH2 domain of the IgG and to the unit of the VH domain of the IgG;
- the unit of the VL domain of the IgG is linked to the unit of the CL domain of the IgG;
- the unit of CL domain of the IgG is linked to the CH1 domain of the IgG;
- and
- the unit of the antigen is linked:
- (1) to the CH3 domain of IgG at the C-terminus and the epitope tag is linked to the C-terminus of the antigen;
- or
- (2) to the unit of the VH domain of the IgG at the N-terminus and the epitope tag is linked to the C-terminus of the CH3 domain of the IgG.
16 more elements
Dependencies are inferred from the claim text.