Method for producing a modified bacteriophage without genome modification
AssigneeTechnische Universität München
InventorsKilian Vogele, Friedrich Simmel, Quirin Emslander
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Abstract
The invention relates to a method for producing a modified bacteriophage in a cell-free expression system wherein the expression of at least one gene of interest is suppressed by a molecule specifically inhibiting its expression. The invention further relates to a composition and a kit for producing a modified bacteriophage. Moreover, the invention relates to a bacteriophage which is not modified on the genomic level but on the proteomic level and its use for therapy, for diagnostic and detection assays.
Claims11
A method for producing a modified bacteriophage in a cell-free expression system comprising the steps of:
- contacting a cell lysate of a microorganism with a genome of the bacteriophage;
- and
- suppressing expression of at least one gene of interest encoded by the genome of the bacteriophage by adding a molecule specifically inhibiting the expression of the endogenous version of the at least one gene of interest;
- and
- expressing a modified version of the at least one gene of interest by adding a molecule encoding a modified version of the gene of interest, and wherein
- the molecule inhibiting the expression of the endogenous version of the at least one gene of interest is a DNAi molecule binding to the ribosome binding side of the gene of interest, and wherein the DNAi molecule does not bind to the ribosome binding site of the modified version of the gene of interest.
Dependencies are inferred from the claim text.