Confined migration microfluidic device for cell culture and drug screening

A confined migration microfluidic device for cell culture and drug screening, comprising:

• at least one microfluidic chip, each of the at least one microfluidic chip comprising:
• a plurality of first channels;
• a plurality of second channels each being disposed in parallel with one of the first channels thereby providing a plurality of pairs of the first channels and the second channels;
• a plurality of confined migration channels;
• a plurality of two extension channels;
• each of the plurality of pairs of the first channels and the second channels being connected through the confined migration channels, each of the confined migration channels having a depth smaller than that of each of the first channels and second channels;
• two opposite ends of each of the first channels being provided with a first inlet and a first outlet, respectively;
• two opposite ends of each of the second channels being provided with a second inlet and a second outlet, respectively;
• and
• an upper chip and a lower chip,
• wherein the plurality of confined migration channels between each of the plurality of pairs of the first channel and the second channel being identical in terms of shape and dimension;
• the plurality of two extension channels each composed of a first extension channel and a second extension channel being respectively disposed at two opposite ends of each of the confined migration channels;
• the first extension channel communicating with the first channel and the confined migration channel;
• the second extension channel communicating with the second channel and the confined migration channel;
• each of the first extension channel and the second extension channel having a depth greater than that of the confined migration channel;
• and
• wherein the upper chip is provided therethrough with the first inlet, the second inlet, the first outlet, and the second outlet, and wherein the upper chip and the lower chip are associated with each other to form each of the at least one microfluidic chip, and wherein the first channels, the second channels, the first extension channels, the second extension channels, and the confined migration channels are formed between the upper chip and the lower chip, and wherein the upper chip comprises upper channel portions;
• the lower chip comprises lower channel portions, and wherein the upper channel portions and the lower channel portions are combined to form all channels, and wherein the upper chip further comprises an upper set of the first channels, an upper set of the second channels, and an upper set of the confined migration channels;
• the lower chip further comprises a lower set of the first channels, a lower set of the second channels, and a lower set of the confined migration channels, and wherein a sum of a depth of each of the upper first channels and that of each of the lower first channels is equal to a sum of a depth of each of the first channels;
• a sum of a depth of each of the upper second channels and that of each of the lower second channels is equal to a sum of a depth of each of the second channels;
• a sum of a depth of each of the confined migration channels of the upper set of the confined migration channels and that of each of the confined migration channels of the lower set of the confined migration channels is equal to a sum of a depth of each of the confined migration channels.