Microfluidic platform for enabling cell culturing in a three dimensional microenvironment

A microfluidic platform for reproduction of cells in the microfluidic platform, comprising

• at least one central area for receiving a solution in the at least one central area, wherein the at least one central area comprises at least one first feeding channel for feeding the solution,
• at least two side areas provided mutually on opposite sides of the at least one central area, each one of the at least two side areas has at least one second feeding channel for feeding a material, wherein
• one of the at least two side areas comprises three or more pockets suitable for accumulation of tumor cells and reproduction of tumor spheroids,
• a height of one side area of the at least two side areas is greater than a height of the at least one central area,
• the insides of the at least one central area and the at least two side areas are primarily filled with air such that a solution fed into the at least one central area reaches the at least two side areas, and then ends its progress due to the surface tension and capillary effect when it meets air therein, and therefore does not overflow the at least two side areas;
• and
• the at least one central area is treated with poly-D-lysine (PDL).