Modified T cell, preparation method therefor and use thereof
AssigneeInstitute of Zoology, Chinese Academy of Sciences
InventorsHaoyi Wang, Xingying Zhang, Chen Cheng, Na Tang, Na Li
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Abstract
The present invention relates to the field of gene editing and tumor immunotherapy. In particular, the invention relates to methods for preparing modified T cells, such as CAR-T cells, by gene editing, and modified T cells prepared by the methods and uses thereof.
Claims9
A method for preparing a modified T cell, comprising a step of eliminating the expression of at least one inhibitory protein in the T cell and a step of eliminating the expression of PD1 in the T cell, wherein the inhibitory protein is a T cell surface inhibitory receptor and/or a T cell exhaustion-related protein, wherein the inhibitory protein is a TGFBRII receptor, wherein said elimination of at least one inhibitory protein and elimination of the expression of PD1 is achieved by antisense RNA, antagomir, siRNA, shRNA, meganuclease, zinc finger nuclease, transcription activator-like effector nuclease, or a CRISPR system, wherein said T cell is a T cell comprising a chimeric antigen receptor (CAR).
Dependencies are inferred from the claim text.