Eluate collection during antibody chromatography

A method for the purification of an antibody by chromatography, comprising the following steps:

• a) loading a sample comprising an antibody onto a chromatography resin,
• b) optionally, washing the resin,
• c) applying an elution buffer to the chromatography resin, and
• d) starting collection of the eluate,
• wherein the collection of the eluate is started at a predetermined interval (D0) after the absorbance signal of the eluate has reached a predetermined value (A0), and wherein the predetermined value (A0) is in the range of 10-50% of the absorbance signal at peak maximum of elution peaks obtained by different elution runs varying in pH conditions within a range of pH 5 to pH 7 and load density within a range of 5 to 50 g/l resin load with the antibody sample to be purified.