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US 12,760,053 B2Granted
Process for the purification of an acidic human milk oligosaccharide from fermentation broth
AssigneeFrieslandCampina Nederland B.V.
InventorsAlfred Willy Bonte, Franciscus Johannes Hubertus Jeurissen, Martina Henrica Maria Van Genuchten
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Abstract
The present invention relates to a process for the purification of an acidic human milk oligosaccharide (HMO) from a fermentation broth using ion exchange methods. This process allows for a reduction of the number and/or extent of desalting operations, such as electrodialysis. It is even possible to refrain from such operations.
Claims19
A process for the purification of an acidic human milk oligosaccharide (HMO) from a fermentation broth, the process comprising:
- (i) separating biomass from the fermentation broth to provide a crude solution;
- (ii) subjecting the crude solution to cation exchange using a cation exchange material in the H+ form, thereby obtaining a solution with a pH in the range 1-3;
- (iii) subjecting the solution with pH in the range 1-3 to anion exchange using a weakly basic anion exchange material in the Cl− form, thereby obtaining a solution with a pH in the range 1.5-5.5;
- (iv) subjecting the solution with pH in the range 1.5-5.5 to adsorption using an adsorbent material in order to remove neutral organic compounds;
- (v) optionally adjusting the pH of the solution having been subjected to adsorption to a value in the range 5-6;
- (vi) optionally subjecting the solution to anion exchange using an anion exchange resin, thereby binding the acidic human milk oligosaccharide to the resin followed by eluting the acidic human milk oligosaccharide from the resin using a salt solution, this optional step being conducted between steps (iii) and (iv) or steps (iv) and (v);
- thereby obtaining a purified solution comprising said acidic human milk oligosaccharide.
4 more elements
Dependencies are inferred from the claim text.