Composition for treating wound comprising dermal tissue-derived extracellular matrix and method for preparing same

A composition for treating wounds consisting essentially of:

• a first biopolymer crosslinked to a dermal extracellular matrix (ECM);
• and
• a second biopolymer;
• wherein:
• the first biopolymer is selected from the group consisting of collagen, hyaluronic acid, chitosan, carboxymethylcellulose, alginate, gelatin, and hydroxyapatite;
• the second biopolymer is selected from the group consisting of collagen, hyaluronic acid, chitosan, carboxymethylcellulose, alginate, gelatin, and hydroxyapatite, or the second biopolymer is a crosslinked product of a biopolymer selected from the group consisting of collagen, hyaluronic acid, chitosan, carboxymethylcellulose, alginate, gelatin, and hydroxyapatite;
• the content of the first biopolymer crosslinked to a dermal extracellular matrix is 5 parts to 40 parts by weight with respect to the total weight of the composition, and the composition for treating wounds has a complex viscosity of 1,000 Pa S to 10,000 Pa S;
• wherein the first biopolymer crosslinked to a dermal extracellular matrix is prepared by:
• i) removing a lipid component from skin tissue of a human;
• ii) preparing dermal ECM by removing cells from adipose tissue from which the lipid component is removed;
• iii) freeze-drying the adipose tissue from which cells are removed;
• iv) powdering the freeze-dried lyophilizate;
• v) preparing a first biopolymer crosslinked to a dermal extracellular matrix by crosslinking the powdered dermal ECM and the first biopolymer;
• vi) freeze-drying the crosslinked dermal ECM-first biopolymer product;
• and
• vii) powdering the freeze-dried lyophilizate;
• viii) preparing the second biopolymer by crosslinking the biopolymer using a crosslinking agent;
• and drying the resulting crosslinked product, wherein the crosslinking agent of the first biopolymer and the second biopolymer is selected from the group consisting of 1,4-butandiol diglycidyl ether (BDDE), ethylene glycol diglycidyl ether (EGDGE), 1,6-hexanediol diglycidyl ether, propylene glycol diglycidyl ether, polypropylene glycol diglycidyl ether, polytetramethylene glycol diglycidyl ether, neopentyl glycol diglycidyl ether, polyglycerol polyglycidyl ether, diglycerol polyglycidyl ether, glycerol polyglycidyl ether, tri-methylpropane polyglycidyl ether, 1,2-(bis(2,3-epoxypropoxy)ethylene, pentaerythritol polyglycidyl ether, and sorbitol polyglycidyl ether;
• and
• ix) mixing the crosslinked dermal extracellular matrix (ECM)-first biopolymer product and the second crosslinked biopolymer.