Method for droplet loading into nanowells
AssigneeAbbott Laboratories
InventorsKaren Leirs, Elena Pérez-Ruiz, Jeroen Lammertyn
Life of this patent
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Abstract
Provided herein is a method of loading wells with a liquid droplet, or a portion thereof, wherein each liquid droplet comprises solid supports and a detergent or surfactant, such that the detergent or surfactant reduces the contact angle between the liquid droplet and the wells. Also provided is a method of detecting and quantifying an analyte of interest in a sample, which involves loading wells in an array with a liquid droplet according to aforementioned method, wherein the liquid droplet comprises an analyte captured on a solid support.
Claims17
A method of loading wells with liquid droplets, which method comprises:
- (a) contacting an array of wells with a liquid droplet, wherein (i) one or more wells of the array is of sufficient size to have loaded therein a portion of the liquid droplet, and (ii) each portion of the liquid droplet comprises a solid support and a detergent or surfactant wherein the detergent is TWEEN-20 or TWEEN-80, and the surfactant is TRITON X-100,
- (b) moving the liquid droplet over the array of wells, wherein the detergent or surfactant reduces the contact angle between the liquid droplet and the wells, and whereby a portion of the liquid droplet is loaded into each well of the array;
- (c) removing any portion of the liquid droplet that is not loaded into wells from the surface of the array;
- and
- (d) sealing the loaded wells.
Dependencies are inferred from the claim text.