Method and device for delivering biological material to a medium

A system for delivering biological material to a medium comprising:

• a container comprising the medium;
• a capsule comprising a cap portion configured to removably secure the capsule to a mouth of the container and an elongate portion comprising a cavity defined at least in part by a frangible bottom, wherein the cavity comprises biological material therein;
• and
• a plunger comprising:
• a first end and a second end;
• one or more walls defining an internal volume of the plunger, the one or more walls moveable along an inner surface of the capsule when the plunger is depressed;
• and
• a shaft disposed at least partially within the internal volume and connected to the second end of the plunger, the shaft including a disrupting mechanism at the first end of the plunger and the disrupting mechanism positioned external to the internal volume of the plunger, wherein the disrupting mechanism is configured to break the frangible bottom when the plunger is depressed;
• wherein the first end of the plunger extends within the cavity of the capsule, and wherein the second end of the plunger extends outward from the cavity and the elongate portion of the capsule.

A method for delivering biological material to a medium comprising:

• securing a capsule to a mouth of a container, the capsule comprising a cap portion configured to removably secure the capsule to a mouth of the container and an elongate portion comprising a cavity defined at least in part by a frangible bottom, wherein the cavity comprises biological material therein;
• suspending the elongate portion of the capsule into a cavity of the container;
• depressing a plunger, the plunger comprising:
• a first end and a second end;
• one or more walls defining an internal volume of the plunger, the one or more walls moveable along an inner surface of the capsule;
• and
• a shaft disposed at least partially within the internal volume and connected to the second end of the plunger, the shaft including a disrupting mechanism at the first end of the plunger and the disrupting mechanism positioned external to the internal volume of the plunger;
• wherein the first end of the plunger extends within the cavity of the capsule, and wherein the second end of the plunger extends outward from the cavity and the elongate portion of the capsule;
• disrupting the frangible bottom of the capsule;
• and
• releasing the biological material into the container.