Ultrasound stimulation of musculo-skeletal tissue structures

A method for ultrasound stimulation of musculo-skeletal tissue structures, the method comprising the steps of:

• generating a sinusoidal-complex in an acoustic spatial-temporal shear mode, wherein the sinusoidal-complex in the acoustic spatial-temporal shear mode is represented as
• s
• ⁡
• (
• t
• )
• AM
• =
• (
• 1
• +
• m
• ⁢
• ⁢
• sin
• ⁢
• ⁢
• ω
• m
• ⁢
• t
• )
• ⁢
• ⁢
• sin
• ⁢
• ⁢
• ω
• c
• ⁢
• t
• =
• sin
• ⁢
• ⁢
• ω
• c
• ⁢
• t
• -
• (
• m
• ⁢
• /
• ⁢
• 2
• )
• ⁢
• ⁢
• cos
• ⁡
• (
• ω
• c
• +
• ω
• m
• )
• ⁢
• t
• +
• (
• m
• ⁢
• /
• ⁢
• 2
• )
• ⁢
• ⁢
• cos
• ⁡
• (
• ω
• c
• -
• ω
• m
• )
• t,
• wherein s(t)AM is a transmitting signal, t is time, ωc is a carrier frequency, m is a modulation index that controls a degree of amplitude modulation, and ωm is a modulation frequency;
• and
• beam-steering the sinusoidal-complex along a bone axis of a musculo-bone fracture tissue in a multi-layered biological tissue structure;
• wherein the beam-steered sinusoidal complex in the acoustic spatial-temporal shear mode activates an integrin response of a bone tissue extracellular matrix through shear-wave stimulation;
• wherein the beam-steered sinusoidal complex in the acoustic spatial-temporal shear mode produces an effective amount of shear stress levels in the multi-layered biological tissue structure that biophysically stimulates bone tissue repair and bone fracture healing in the musculo-bone fracture tissue, beginning with periosteal healing in the musculo-bone fracture tissue,
• wherein a transmitting signal amplitude modulation envelope of the sinusoidal-complex in the acoustic spatial-temporal shear mode is constant, an osteogenic acoustic intensity shear stress level per beam of the sinusoidal-complex in the acoustic spatial-temporal shear mode is 50 milliwatts/cm2, a pulse repetition frequency (PRF) of the sinusoidal-complex in the acoustic spatial-temporal shear mode for bone tissue repair is 1 kilohertz (kHz), a duty cycle of the sinusoidal-complex in the acoustic spatial-temporal shear mode is about 20%, a frequency of the sinusoidal-complex in the acoustic spatial-temporal shear mode for long bone healing is 1.0 megahertz (MHz) and a frequency of the sinusoidal-complex in the acoustic spatial-temporal shear mode for spinal fusion healing is 0.5 megahertz MHz, and a dosage time of the sinusoidal-complex in the acoustic spatial-temporal shear mode is equal to or less than 20 minutes, and
• wherein the osteogenic acoustic intensity shear stress level, PRF, the duty cycle, the frequency for the long bone healing, the frequency for the spinal fusion healing, and the dosage time have been simultaneously optimized to produce the amount of the shear stress levels in the multi-layered biological tissue structure that biophysically stimulates the bone tissue repair and the bone fracture healing in the musculo-bone fracture tissue.