Polymer coatings
AssigneeIllumina, Inc.
InventorsWayne N. George, Andrew A. Brown, Daniel Bratton, Hongji Ren, Ryan Christopher Smith
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Abstract
The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.
Claims20
A substrate comprising a polymer coating covalently attached to a surface of the substrate, wherein the polymer coating comprises recurring units of Formula (I):
- wherein each R1 and R1′ is independently hydrogen or alkyl;
- R2 is H;
- and
- R3 is substituted alkylamido, or substituted alkylthio wherein R3 comprises a reactive group selected from the group consisting of optionally substituted alkene, optionally substituted alkyne, oxo-amine, azido, formyl, hydroxyl, hydrazinyl, hydrazonyl, cyanuric chloride, thiocyanate, carboxyl, glycidyl, activated ester, epoxy, aziridine, triazoline, and thiol.
A method of immobilizing a polymer to a surface of a substrate, comprising:
- contacting a polymer with a surface of a substrate, wherein the surface comprising a series of functional groups attached thereto, and the polymer comprises recuring-recurring units of Formula (I):
- wherein each R1 and R1′ is independently hydrogen or alkyl;
- R2 is H;
- R3 is substituted alkylamido, or substituted alkylthio, wherein R3 comprises a reactive group selected from the group consisting of optionally substituted alkene, optionally substituted alkyne, oxo-amine, azido, formyl, hydroxyl, hydrazinyl, hydrazonyl, cyanuric chloride, thiocyanate, carboxyl, glycidyl, activated ester, epoxy, aziridine, triazoline, and thiol;
- and
- reacting the reactive groups of the polymer with the series of functional groups attached to the surface of the substrate.
Dependencies are inferred from the claim text.