Filtration systems, components and methods

A tangential flow filtration (TFF) system providing a disposable flow path for processing a fluid feed material, the system comprising a disposable reactor vessel and a disposable TFF flow path comprising a disposable single use TFF filtration unit, wherein:

• the disposable reactor vessel comprises feed material and/or buffer and is connected through disposable low-pressure tubing to a feed pump;
• the disposable single use TFF filtration unit comprises:
• a bottom support base comprising a manifold supply inlet and a permeate drain, and
• a top support base comprising a manifold outlet and a permeate outlet, and, cassette filters;
• the feed pump is further connected through disposable high-pressure tubing to the manifold supply inlet;
• a recirculation pump connected to a recirculation loop comprising a supply side conduit connected to the manifold supply inlet and a return side conduit connected to the manifold outlet, the supply side and return side conduits being comprised of disposable high-pressure tubing;
• the return side conduit comprises a retentate return outlet for return of the feed and/or retentate to the disposable reactor vessel through low-pressure disposable tubing;
• the disposable reactor vessel is not directly connected to the high-pressure disposable tubing;
• the disposable reactor vessel, disposable low-pressure tubing, disposable high-pressure tubing, and disposable single use TFF filtration unit are comprised of plastic, thermoplastic elastomer, rubber, high-density polyethylene, a combination of low-density and high-density polyethylene, or nylon;
• and,
• the disposable high-pressure tubing has a Shore durometer of at least 40 D or is braided reinforced silicon.