Processes for producing fermentation products
AssigneeNovozymes A/S
InventorsRandall Scott Deinhammer, Joyce Craig, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong, Zhengfang Kang
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Abstract
The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.
Claims27
A cellulolytic composition comprising:
- (a) a beta-glucosidase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 22 and having the substitutions F100D, S283G, N456E and F512Y;
- and
- (b) a cellobiohydrolase I (“CBHI”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 24;
- and
- (c) a cellobiohydrolase II (“CBHII”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 25;
- and
- a glucoamylase.
A process for producing ethanol from corn, the process comprising:
- (i) liquefying the corn at a temperature above the initial gelatinization temperature using a thermostable alpha-amylase and a thermostable protease;
- (ii) saccharifying using a glucoamylase;
- and
- (iii) fermenting with a yeast, wherein a cellulolytic composition is present or added during saccharifying step (ii) and/or fermenting step (iii), wherein the cellulolytic composition comprises:
- (a) a beta-glucosidase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 22 and having the substitutions F100D, S283G, N456E and F512Y;
- and
- (b) a cellobiohydrolase I (“CBHI”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 24;
- and
- (c) a cellobiohydrolase II (“CBHII”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 25.
Dependencies are inferred from the claim text.