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US 12,636,304 B2Granted
Metabolically stabilized double stranded mRNA
AssigneeUniversity of Iowa Research Foundation
InventorsKevin G. Rice, Samuel T. Crowley
Life of this patent
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Abstract
Double stranded mRNA, e.g., produced in vitro, as well as method of making and using the ds mRNA, are provided. For example, the disclosure provides a method of expressing a prophylactic or therapeutic protein in mammalian cells in vivo, that includes introducing a composition comprising ds mRNA that encodes a protein to the mammalian cells in an amount effective to express the protein in vivo.
Claims16
A method to induce an immune response in a mammal, comprising:
- administering to the mammal a composition comprising a double-stranded (ds) mRNA encoding a protein, wherein the protein is an antigen, wherein the ds mRNA comprises a forward strand and a reverse strand, wherein the forward strand of the ds mRNA has a 5′ cap, a start codon, a poly A sequence and encodes the protein, wherein the reverse strand comprises a 5′ amino allyl modified uridine or cytidine, wherein the 5′ amino allyl modified uridine or cytidine is functionalized with an anhydride, or an N-hydroxysuccinamide ester, wherein the two strands of the ds mRNA are hydrogen bonded over at least 50% of the length of the strands, wherein the hydrogen bonded strands include at least a portion of the coding region for the protein;
- wherein following administration of the composition to the mammal, the mammal expresses the protein, and wherein the protein induces an immune response in the mammal;
- wherein the composition further comprises a carrier protein or a synthetic polymer.
Dependencies are inferred from the claim text.