Method and apparatus for analyzing biochip image, computer device, and storage medium

A method for analyzing a biochip image, comprising:

• acquiring and preprocessing the biochip image to obtain a preprocessed image;
• performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image;
• and
• performing an enhancement processing on the deflection-corrected image, and identifying a positive or negative of an area of interest in the preprocessed image according to an image on which the enhancement processing has been performed;
• wherein the performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image comprises:
• selecting a preset number of detection areas in the preprocessed image;
• detecting a center and a radius of each area of interest in each detection area by using a Hough circle transformation;
• and
• forming a circle according to the center and the radius of each area of interest, to determine the area of interest and segment the area of interest;
• wherein the performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image comprises:
• performing an expansion processing on the segmented image to connect adjacent areas of interest in a preset direction;
• performing a principal component analysis on a contour of the detection area having a maximum contour in the image on which the expansion processing is performed, to obtain a contour direction;
• and
• determining an image deflection angle according to the contour direction, to correct the preprocessed image and to obtain the deflection-corrected image;
• wherein the performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image comprises:
• enlarging the selected area by a preset proportion, to randomly select the preset number of detection areas in the preprocessed image again;
• and
• repeatedly and iteratively detecting the image deflection angle until the image deflection angle is smaller than a preset angle threshold, to obtain the deflection-corrected image;
• wherein a range of the preset angle threshold is determined by a following conditional expression:
• cos
• ⁢
• θ
• -
• (
• max
• ⁢
• {
• m
• ,
• n
• }
• -
• 1
• )
• ·
• sin
• ⁢
• θ
• >
• 2
• ⁢
• rad
• dist
• where θ is the preset angle threshold, dist is the area interval of the areas of interest, rad is an area radius of the area of interest, m is the number of rows of the areas of interest in each detection area, and n is the number of columns of the areas of interest in each detection area.