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US 12,601,683 B2Granted
Serial multichannel microscopy
AssigneeMiltenyi Biotec GmbH
InventorWerner Muller
Life of this patent
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Abstract
The invention is directed to a much faster way of combining multiple colour channels in fluorescence microscopy for complex fast diagnostic and research purposes. The advantage is that the procedure and the microscope setting can be simplified and miniaturised to an extend that the construction of such automatic systems become much easier and by omitting the erasing step the speed of analysis is faster and moved to the processing of the images by image processing systems, nowadays working at almost real time.
Claims11
A method for detecting target moieties in a sample of biological specimen by:
- a) contacting the sample with a first conjugate comprising a first antigen recognizing moiety Y and a first fluorescent moiety X, thereby binding at least a part of the first conjugate to the target moieties recognized by the first antigen recognizing moiety Y
- b) removing the first conjugates not bound to the target moieties from the sample
- c) obtaining a first image of the sample thereby detecting the target moieties labelled with the first conjugate;
- and
- d) repeating steps a) to c) with at least one second conjugate comprising a second antigen recognizing moiety Y′ and a second fluorescent moiety X′, thereby obtaining at least one second image and wherein the first and second antigen recognizing moieties Y and Y′ bind to different target moieties characterized in that the intensity of the first image is reduced by a degradation function and then subtracted from the at least one second image.
2 more elements
Dependencies are inferred from the claim text.