Multiple KASP marker primer set for wheat plant height major genes and use thereof
AssigneeJiangsu Academy of Agricultural Sciences
InventorsPeng Jiang, Xu Zhang, Lei Wu, Yi He, Chang Li
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Abstract
A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.
Claims4
A multiple primer set for Kompetitive Allele Specific PCR (KASP) assay for simultaneously detecting Rht-B1 and Rht-D1 genes controlling wheat plant height, wherein the primer set consists of a forward primer F with a nucleotide sequence consisting of SEQ ID NO. 10, a forward primer H with a nucleotide sequence consisting of SEQ ID NO. 11, and a reverse universal primer R with a nucleotide sequence consisting of SEQ ID NO. 9,
- wherein the forward primer F comprises a first fluorescent label-binding sequence at its 5′ end, and targets a single nucleotide polymorphism (SNP) in the Rht-B1 gene,
- wherein the forward primer H comprises a second fluorescent label-binding sequence at its 5′ end, and targets an SNP in the Rht-D1 gene,
- wherein the second fluorescent label-binding sequence is different from the first fluorescent label-binding sequence,
- wherein the reverse universal primer R is shared by the forward primer F and the forward primer H to enable simultaneous PCR amplification from both the Rht-B1 and Rht-D1 genes in a single reaction.
Dependencies are inferred from the claim text.