Compositions and methods for controlling production of polypeptides in cells
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsKarl A. Deisseroth, Charu Ramakrishnan, Yoon Seok Kim, Lief E. Fenno
Life of this patent
Estimating the term…
Abstract
The present disclosure provides recombinant expression vectors for modulating production of polypeptides of interest in a target cell or target cell population. Aspects of the disclosure include recombinant expression vectors having coding sequences encoding portions of a polypeptide of interest, where the coding sequences are flanked by recombinase recognition sites. Also provided are methods for using the recombinant expression vectors as well as a device for monitoring expression of the polypeptide of interest.
Claims25
A recombinant expression vector comprising:
- a) a first coding sequence encoding a portion of a polypeptide of interest, wherein a first recombinase recognition site is positioned 5′ to the first coding sequence;
- b) a second coding sequence positioned 3′ to the first coding sequence, the second coding sequence encoding a portion of the polypeptide of interest;
- c) a first non-coding sequence comprising a first recombinase recognition site and a second recombinase recognition site positioned between the first coding sequence and the second coding sequence;
- d) a third coding sequence positioned 3′ to the second coding sequence, the third coding sequence encoding a portion of the polypeptide of interest, wherein a third recombinase recognition site is positioned 3′ to the third coding sequence;
- and
- e) a second non-coding sequence comprising a second recombinase recognition site and a third recombinase recognition site positioned between the second coding sequence and the third coding sequence.
A method for modulating production of a polypeptide of interest in a target cell or a target cell population, the method comprising:
- introducing a recombinant expression vector comprising
- a) a first coding sequence encoding a portion of a polypeptide of interest, wherein a first recombinase recognition site is positioned 5′ to the first coding sequence;
- b) a second coding sequence positioned 3′ to the first coding sequence, the second coding sequence encoding a portion of the polypeptide of interest;
- c) a first non-coding sequence comprising a first recombinase recognition site and a second recombinase recognition site positioned between the first coding sequence and the second coding sequence;
- d) a third coding sequence positioned 3′ to the second coding sequence, the third coding sequence encoding a portion of the polypeptide of interest, wherein a third recombinase recognition site is positioned 3′ to the third coding sequence;
- and
- e) a second non-coding sequence comprising a second recombinase recognition site and a third recombinase recognition site positioned between the second coding sequence and the third coding sequence
- into the target cell or the target cell population.
Dependencies are inferred from the claim text.