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US 12,600,961 B2Granted
Methods and systems for droplet-based single cell barcoding
Assignee10x Genomics, Inc.
InventorsZahra Kamila Belhocine, Rajiv Bharadwaj, Christopher Hindson, Michael Schnall-Levin, Bill Kengli Lin, Anthony Makarewicz, Pranav Patel, Andrew D. Price, Mohammad Rahimi Lenji, Tobias Daniel Wheeler, Yifeng Yin
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Abstract
Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.
Claims19
A method for processing or analyzing one or more components from a sample, comprising:
- (a) subjecting a plurality of cell beads to bulk denaturation, wherein a cell bead of said plurality of cell beads comprises one or more macromolecular constituents, derived from a cell or a virus, encapsulated within a polymer or gel matrix, wherein said one or more macromolecular constituents comprise a nucleic acid molecule, and wherein said bulk denaturation comprises denaturing said nucleic acid molecule to generate a denatured nucleic acid molecule in said cell bead;
- (b) partitioning said plurality of cell beads and a plurality of barcode beads into a plurality of partitions, wherein a barcode bead of said plurality of barcode beads comprises a plurality of nucleic acid barcode molecules for barcoding said nucleic acid molecule, and wherein upon partitioning, a partition of said plurality of partitions comprises said cell bead and said barcode bead.
Dependencies are inferred from the claim text.