US 12,600,951 B2 · Systems and methods for continuous cultivation and recovery of comestible non-human animal cells

Abstract

The present disclosure relates to systems, apparatuses, and methods for continuous cultivation and recovery of comestible non-human animal cells in a suspension bioreactor. For example, in one or more implementations, the disclosed methods include cultivating non-human animal cells in suspension culture within a bioreactor vessel, removing a measure of suspension culture from the bioreactor vessel while continuing to provide cell culture media thereto, and recovering a first portion of cultivated non-human animal cells from the removed measure of suspension culture while returning a second portion of cultivated non-human animal cells to the bioreactor vessel. Also, in some implementations, the disclosed systems include at least one sterile output module configured to prevent backflow contamination when recovering cultivated cells and removing spent media during continuous cultivation of non-human animal cells according to the disclosed methods.

Claims20

Dependent claims

A method for producing comestible non-human animal cells, the method comprising:

cultivating suspension culture comprising non-human animal cells in a bioreactor vessel;
removing a measure of the suspension culture from the bioreactor vessel while providing cell culture media to the bioreactor vessel;
separating the removed measure of the suspension culture into a concentrated cell culture and a culture broth with cells removed;
recovering a first portion of the concentrated cell culture for harvest concurrent with returning a second portion of the concentrated cell culture to the bioreactor vessel without returning the first portion to the bioreactor vessel;
and
processing cultivated non-human animal cells from the first portion of the concentrated cell culture to produce a cell-based food product.

3 more elements

A method for producing comestible non-human animal cells, the method comprising:

growing non-human animal cells in a suspension culture within a bioreactor for a first period of time;
and
cultivating the suspension culture within the bioreactor for a second period of time by:
continuously providing cell culture media to the bioreactor for the second period of time;
drawing a measure of the suspension culture from the bioreactor during the second period of time;
separating the drawn measure of the suspension culture into a concentrated cell culture and a culture broth with cells removed;
returning a first portion of the concentrated cell culture to the bioreactor while continuously providing the cell culture media;
and
recovering, during the second period of time, a second portion of the concentrated cell culture for harvest without returning the second portion to the bioreactor;
and
processing cultivated non-human animal cells of the second portion of the concentrated cell culture to produce a cell-based food product.

8 more elements

Dependencies are inferred from the claim text.

Systems and methods for continuous cultivation and recovery of comestible non-human animal cells

Life of this patent

Systems and methods for continuous cultivation and recovery of comestible non-human animal cells

Life of this patent

Abstract

Claims20