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US 12,600,944 B2Granted
Multiplex genome editing of immune cells to enhance functionality and resistance to suppressive environment
AssigneeBoard of Regents, The University of Texas System
InventorsRafet Basar, Elizabeth Shpall, Katy Rezvani
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Abstract
Provided herein are methods for producing immune cells with disruption of multiple genes. Further provided are methods for inserting a chimeric antigen receptor at a gene locus of an immune cell.
Claims14
An engineered natural killer (NK) cell with knockout of at least two gene products,
- wherein the at least two gene products are selected from the group consisting of NK Cell Receptor A (NKG2A) and Cytokine Inducible SH2-Containing Protein (CISH),
- wherein knockout comprises the elimination or reduction of level of expression of the at least two gene products, compared to the level of expression of the at least two gene products in the absence of the knockout.
An engineered NK cell with knockout of at least two gene products,
- wherein the at least two gene products are
- Transforming Growth Factor Beta Receptor 2 (TGFBR2) and Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1)
- wherein knockout comprises the elimination or reduction of level of expression of the at least two gene products, compared to the level of expression of the at least two gene products in the absence of the knockout,
- wherein the engineered NK cell is engineered to express a CAR, wherein the CAR comprises one or more antigen-binding domain that targets one or more tumor associated antigens.
1 more element
Dependencies are inferred from the claim text.