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US 12,600,935 B2Granted
Device for forming and counting spheroids and method for producing same, and spheroid culturing method and counting method using same
AssigneesKorea Research Institute of Chemical Technology, The Industry & Academic Cooperation in Chungnam National University (IAC)
InventorsSun Woong Kang, Kang Moo Huh, Sung Hwan Moon, Da Eun Kim, Hye Min Oh, Hye Eun Shim
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Abstract
A device for forming and counting spheroids according to one embodiment of the present invention may comprise a substrate, and a mesh member which is configured to be able to be positioned on the surface of the substrate and has a plurality of protruding members. The plurality of protruding members intersects one another in the form of a mesh so as to form a plurality of pores.
Claims9
A device for forming and counting spheroids, comprising:
- a flat substrate;
- and
- a flexible mesh member, configured with a plurality of protruding members intersecting parallel to each other in a mesh configuration to form a plurality of pores,
- wherein the substrate and the mesh member are each coated with hexanoyl glycol chitosan;
- wherein each pore is configured to have a flat bottom surface and a suitable size to accommodate one spheroid of uniform size;
- wherein the mesh member is fixed by an assembly member positioned at the side of the substrate to place the mesh member in a position above the surface of the substrate and to provide a gap between the mesh member and the surface of the substrate;
- and
- wherein the gap between the mesh member and the surface of the substrate is smaller than the size of the spheroid.
5 more elements
Dependencies are inferred from the claim text.