Microfluidic cell culture plate for air-liquid interface and 3D cultured tissue applications

A culture plate, comprising:

• a plurality of well reservoirs;
• and
• a plurality of microfluidic devices, each of the plurality of microfluidic devices comprising:
• a first channel having a first inlet port and a second inlet port, sidewalls of the first channel defined in a first layer, a bottom surface of the first channel defined by an optically clear layer coupled to the first layer, each of the first inlet port and the second inlet port defined at least in part in a second layer;
• a membrane layer having a first surface coupled to the first layer defining the first channel and a second surface coupled to the second layer, the membrane layer comprising a semipermeable membrane that forms a top surface of the first channel, the membrane layer comprising a first opening aligned with the first inlet port and a second opening aligned with the second inlet port;
• and
• a chamber defined as a slot in the second layer coupled to the second surface of the membrane layer, the chamber having a length greater than at least twice a width of the chamber, the chamber comprising a first divot defined in the second layer at a first end of the chamber and a second divot defined in the second layer at a second end of the chamber, the chamber exposing a portion of the second surface of the membrane layer to an external environment via an opening in the second layer, wherein the chamber overlaps a portion of the first channel across the membrane layer.