Monoclonal antibodies and methods of use
AssigneeElanco Tiergesundheit AG
InventorsSarah Rue, Brendan Eckelman, Quinn L. Deveraux, Marc Nasoff
Life of this patent
Estimating the term…
Abstract
This disclosure relates to immunogens and monoclonal antibodies useful in the identification and/or treatment of cancer cells, including those of the dog. In one example, chimeric anti-canine CD20 antibodies are provided. The antibodies can be used therapeutically to treat lymphoma in dogs.
Claims22
An antibody comprising at least one heavy chain variable region polypeptide comprising
- a heavy chain (H) complementary determining region (CDR) 1 (CDRH1) comprising DYGML (SEQ ID NO.:
- 20), a CDRH2 comprising YISSGSSTIYYADRVKG (SEQ ID NO.:
- 22), a CDRH3 comprising GTFAY (SEQ ID NO.:
- 24), and
- a light chain (L) CDR 1 (CDRL1) comprising RSSQSLIYNNGNTYLH (SEQ ID NO.:
- 70), a CDRL2 comprising KVSNRFS (SEQ ID NO.:
- 27), and a CDRL3 comprising SQSTHVPFT (SEQ ID NO.:
- 29).
A chimeric antibody comprising a light chain variable region (VL) sequence and a heavy chain variable region (VH) sequence consisting of:
- a. a light chain variable region (VL) sequence SEQ ID NO.:3 having an amino acid substitution of N33K, and a heavy chain variable region sequence (VH) SEQ ID NO.:6;
- and
- wherein the antibody binds canine CD20.
An isolated nucleic acid encoding an antibody comprising:
- a. a heavy chain (H) complementary determining region (CDR) 1 (CDRH1) comprising DYGML (SEQ ID NO.:
- 20), a CDRH2 comprising YISSGSSTIYYADRVKG (SEQ ID NO.:
- 22), a CDRH3 comprising GTFAY (SEQ ID NO.:
- 24), and
- a light chain (L) CDR 1 (CDRL1) comprising RSSQSLIYNKGNTYLH (SEQ ID NO.:
- 70), a CDRL2 comprising KVSNRFS (SEQ ID NO.:
- 27), and a CDRL3 comprising SQSTHVPFT (SEQ ID NO.:
- 29),
- wherein the antibody binds canine CD20.
A kit for identifying, detecting or targeting cells expressing canine CD20 comprising
- a heavy chain (H) complementary determining region (CDR) 1 (CDRH1) comprising DYGML (SEQ ID NO.:
- 20), a CDRH2 comprising YISSGSSTIYYADRVKG (SEQ ID NO.:
- 22), a CDRH3 comprising GTFAY (SEQ ID NO.:
- 24), and
- a light chain (L) CDR 1 (CDRL1) comprising RSSQSLIYNKGNTYLH (SEQ ID NO.:
- 70), a CDRL2 comprising KVSNRFS (Seq ID NO.:
- 27), and a CDRL3 comprising SQSTHVPFT (SEQ ID NO.:
- 29),
- wherein the antibody binds canine CD20;
- and instructions for use and/or one or more other reagents selected from buffers, blocking agents and detection reagents.
Dependencies are inferred from the claim text.