Therapeutic RNA for HPV-positive cancer

A composition or medical preparation comprising at least one RNA, wherein the at least one RNA encodes:

• (i) an amino acid sequence comprising human papillomavirus (HPV) E6 protein, an immunogenic variant thereof, or an immunogenic fragment of the HPV E6 protein or the immunogenic variant thereof;
• and
• (ii) an amino acid sequence comprising HPV E7 protein, an immunogenic variant thereof, or an immunogenic fragment of the HPV E7 protein or the immunogenic variant thereof;
• wherein (a) the RNA encoding the amino acid sequence under (i) comprises at least one of:
• the nucleotide sequence of SEQ ID NO:
• 2, a nucleotide sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, or 80% identity to the nucleotide sequence of SEQ ID NO:
• 2,
• a nucleotide sequence encoding the amino acid sequence of amino acids 37 to 194 of SEQ ID NO:
• 1, and a nucleotide sequence encoding an amino acid sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, or 80% identity to the amino acid sequence of amino acids 37 to 194 of SEQ ID NO:
• 1;
• or
• (b) the RNA encoding the amino acid sequence under (ii) comprises at least one of:
• the nucleotide sequence of SEQ ID NO:
• 4, a nucleotide sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, or 80% identity to the nucleotide sequence of SEQ ID NO:
• 4,
• a nucleotide sequence encoding the amino acid sequence of amino acids 37 to 134 of SEQ ID NO:
• 3, and a nucleotide sequence encoding an amino acid sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, or 80% identity to the amino acid sequence of amino acids 37 to 134 of SEQ ID NO:
• 3.