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US 12,599,642 B2Granted
Compositions derived from Salvia hispanica seeds
AssigneeSignum Biosciences, Inc.
InventorsJose Fernandez, Eddie Perez, Kristen Huber, Jason Healy, Corey Webb, Karl Rouzard, Mike Voronkov, George Nikonov
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Abstract
Methods of preparing a composition from chia seeds are provided. Compositions, including compositions prepared by the presently disclosed methods, and methods regarding selecting chia seeds and administering chia seed extracts are also disclosed.
Claims18
A method of preparing an extract from chia seeds, said method comprising:
- a) introducing acetonitrile to chia seeds to form a mixture;
- b) agitating the mixture at a maintained temperature above room temperature for a period of time;
- c) filtering the chia seeds from the agitated mixture;
- d) evaporating the filtered mixture under reduced pressure to remove the acetonitrile and to form a crude chia seed oil;
- e) introducing methyl t-butyl ether to the crude chia seed oil to form a solution;
- f) adding a decolorant to the solution;
- g) agitating the solution containing the decolorant for a second period of time;
- h) filtering the decolorant from the agitated solution;
- and
- i) evaporating the methyl t-butyl ether from the filtered solution under reduced pressure, thereby preparing the extract,
- wherein relative to the crude chia seed oil, the extract comprises one or more of the following:
- an increased amount of cis, cis-9, 12-octadecadienoic acid (LA);
- an increased amount of cis, cis, cis-9, 12, 15-octadecatrienoic acid (ALA);
- and/or
- increased stability and reduced color change when stored at 50° C. for up to eight weeks.
12 more elements
Dependencies are inferred from the claim text.