Therapeutic agent for nerve disorders

A method of treatment for a nerve disorder of a subject in need thereof, wherein the method comprising:

• nasally administering to the subject an therapeutic agent comprising a culture supernatant of a mesenchymal stem cell and/or a cell capable of differentiating into a mesenchymal stem cell, and is used in combination with stimulation of a nerve of the subject, and
• the stimulation is performed between before and 16 hours after administration of the therapeutic agent.

A method of treatment for a nerve disorder of a subject in need thereof, wherein the method comprising:

• intravenously administering to the subject an therapeutic agent comprising a culture supernatant of a mesenchymal stem cell and/or a cell capable of differentiating into a mesenchymal stem cell, and is used in combination with stimulation of a nerve of the subject, and
• the stimulation is performed between before and 3 hours after administration of the therapeutic agent.

A method of treatment for a nerve disorder of a subject in need thereof, wherein the method comprising:

• subcutaneously administering to the subject an therapeutic agent comprising a culture supernatant of a mesenchymal stem cell and/or a cell capable of differentiating into a mesenchymal stem cell, and is used in combination with stimulation of a nerve of the subject, and
• the stimulation is performed between before and 16 hours after administration of the therapeutic agent.

A method of treatment for a nerve disorder of a subject in need thereof, wherein the method comprising:

• administering to the subject an therapeutic agent comprising a culture supernatant of a mesenchymal stem cell and/or a cell capable of differentiating into a mesenchymal stem cell, and is used in combination with stimulation of a nerve of the subject, and
• the stimulation is preferentially applied to one or more sites selected from the group consisting of a nerve injury site, an area surrounding the nerve injury site, and a site compensating for the function of the nerve injury site, wherein the culture supernatant is not diluted or concentrated in terms of soluble solid content.

A method of treatment for a nerve disorder of a subject in need thereof, wherein the method comprising:

• administering to the subject an therapeutic agent comprising a culture supernatant of a mesenchymal stem cell and/or a cell capable of differentiating into a mesenchymal stem cell, and is used in combination with stimulation of a nerve of the subject, and
• the stimulation is preferentially applied to one or more sites selected from the group consisting of a nerve injury site, an area surrounding the nerve injury site, and a site compensating for the function of the nerve injury site, wherein a lyophilizate of the culture supernatant is dissolved and then used at the time of administration.