Serum exosome with high osteogenesis and high angiogenesis, preparation method, and application thereof

A preparation method of a serum exosome with osteogenesis and angiogenesis derived from a serum of a fracture recovery period, comprising:

• step (1), collecting a blood sample during the fracture recovery period, then separating the blood sample to obtain a serum;
• wherein the fracture recovery period is in a range of a second week to a fifth week after fracture, and the blood sample is a mixed blood sample of peripheral arterial blood collected at the second, third, fourth and fifth weeks of the fracture recovery period;
• step (2), diluting the serum, performing solid-liquid separation on the diluted serum to obtain a supernatant;
• and
• step (3), performing solid-liquid separation on the supernatant to obtain a sediment, wherein the sediment is the serum exosome with osteogenesis and angiogenesis;
• and
• wherein the serum exosome is used for bone defect repair and repair of trauma.