Uric acid liposomes
AssigneesHospital Clinic De Barcelona, Fundació De Recerca ClíNic Barcelona-Institut D'Investigacions BiomèDiques August Pii Sunyer, Agencia Estatal Consejo Superior de Investigaciones Cientificas, M.P.
InventorsAngel Chamorro Sánchez, Ana María Planas Obradors, Pedro Ramos Cabrer
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Abstract
The present invention relates to liposomes that encapsulate uric acid, methods for its preparation and uses of said liposomes.
Claims14
Liposome that encapsulates uric acid, uric acid salts, or combinations thereof, wherein said liposome has a lipid bilayer comprising double-chain phospholipids and positively charged cholesterol, wherein said liposome is unilamellar.
A method for the manufacture of liposomes that encapsulate uric acid, uric acid salts, or combinations thereof, said method comprising the steps of:
- a) preparing a solution comprising lithium and/or potassium ions in water;
- b) adjusting said solution to a pH value between 10 and 11;
- c) adding uric acid, uric acid salts, or combinations thereof, in which the lithium and/or potassium ions are present in an molar amount that is at least twice than of uric acid, keeping the pH in the range of 10-11 to obtain a complete dissolution;
- d) lowering the pH to a value between 7.2 and 7.4 to obtain a uric acid solution;
- e) forming a lipid film;
- f) rehydrating the lipid film formed in step e) with the uric acid solution of step d) to obtain liposomes;
- g) extruding the obtained liposomes in step f) using the lithium and/or potassium ions solution of step a) as solvent;
- and
- h) filtering the obtained liposomes in step g) using the lithium and/or potassium ions solution of step a) as solvent,
- wherein steps e) to h) are carried out free of calcium ions.
Dependencies are inferred from the claim text.