Reference dataset-based, spectrometric characterization of cell substrates using sub-libraries

A method for spectrometrically characterizing a test cell substrate, comprising:

• a) providing a library comprising a multitude of reference data sets, with each reference data set containing data that allows a taxonomic classification of a cell substrate;
• b) obtaining first spectrometric measurement data from the test cell substrate after a first preparation;
• c) comparing the first spectrometric measurement data or data derived therefrom with the library to determine a first match result, wherein a match result contains a list of reference data sets and their degree of matching with spectrometric measurement data of a cell substrate or data derived therefrom;
• d) creating a sub-library comprising reference data sets from the library for which the first match result is evaluated as allowing a taxonomic classification of the test cell substrate;
• e) obtaining second spectrometric measurement data from the test cell substrate after at least a second preparation under conditions that are not identical to the conditions of the first preparation;
• f) comparing the second spectrometric measurement data or data derived therefrom with the sub-library to determine a second match result;
• and
• g) determining a property of the test cell substrate using the second match result.