Expression of products from nucleic acid concatemers

A method comprising:

• formulating a mixture comprising at least a first nucleic acid concatemer, a second nucleic acid concatemer, and a third nucleic acid concatemer in a predefined ratio, wherein each of the nucleic acid concatemers comprises tandem repeats of two or more nucleic acid sequences;
• and
• co-expressing the concatemer mixture to generate a first expression product, a second expression product, and a third expression product from each nucleic acid concatemer in the mixture,
• wherein the first nucleic acid concatemer, the second nucleic acid concatemer, the third nucleic acid concatemer, or any combination thereof are over 10 kb in length,
• wherein the molar ratio of the the first expression product, the second expression product, and the third expression product is proportional to the predefined molar ratio of the first nucleic acid concatemer, the second nucleic acid concatemer, and the third nucleic acid concatemer in the concatemer mixture, and
• wherein the molar ratio of the first concatemer to the second concatemer to the third concatemer is C1:C2:Cn, where C1, C2, and Cn each is between 1 and 10.

A method, comprising:

• amplifying at least one template comprising a first nucleic acid sequence using strand-displacement rolling circle amplification to a generate a first concatemer comprising tandem repeats of the first nucleic acid sequence;
• contacting the first concatemer with a second concatemer comprising tandem repeats of a second nucleic acid sequence to form a concatemer mixture having a predefined ratio of the first nucleic acid concatemer to the second nucleic acid concatemer;
• and
• co-expressing the concatemer mixture to generate a first expression product from the first nucleic acid sequence and a second expression product from the second nucleic acid sequence, wherein a ratio of the first expression product to the second expression product is proportional to the predefined ratio of the first nucleic acid concatemer to the second nucleic acid concatemer in the concatemer mixture,
• wherein the molar ratio of the first concatemer to the second concatemer is between 1:1 and 1:10, and
• wherein the first concatemer, the second concatemer, or both are over 10 kb in length.

A method comprising:

• formulating a mixture comprising at least one nucleic acid concatemer and at least one plasmid having a predefined ratio to one another, wherein the at least one nucleic acid concatemer comprises tandem repeats of a first nucleic acid sequence and wherein the at least one plasmid comprises a second nucleic acid sequence;
• and
• co-expressing the mixture to generate a first expression product from the first nucleic acid sequence and a second expression product from the second nucleic acid sequence,
• wherein the molar ratio of the first concatemer to the plasmid is between 1:1 and 1:10,
• wherein the first nucleic acid concatemer is over 10 kb in length, and
• wherein the co-expression of the first expression product and the second expression product is ratiometric based on the predefined ratio.