US 12,595,480 B2 · Method for producing nucleic acid molecule, biomaterial, and method for producing biomaterial

Abstract

The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of: (A) bringing a candidate nucleic acid molecule into contact with the first biological molecule to select a nucleic acid molecule that has bound to the first biological molecule as a first selected nucleic acid molecule; and (B) selecting the first selected nucleic acid molecule as an intended nucleic acid molecule.

Claims9

Dependent claims

A nucleic acid molecule that binds to VEGF, the nucleic acid molecule comprising:

any one of the following polynucleotides (a) and (b):
(a) a polynucleotide that consists of a base sequence of SEQ ID NO:
1 or a partial sequence of the base sequence of SEQ ID NO:
1;
and
(b) a polynucleotide that consists of a base sequence having at least 80% identity to the base sequence of the polynucleotide (a), binds to VEGF, and does not inhibit a function of the VEGF.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/773,461Filed 29 Oct 2020 · continuation

Priority

WO PCT/JP2020/01814228 Apr 2020

Published

US 2022/0396792 A115 Dec 2022

Granted

7 Apr 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 15/113
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: non-coding nucleic acids modulating the…
C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants
C07K 14/70578
Receptors; Cell surface antigens; Cell surface determinants: nGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
C07K 14/71
Receptors; Cell surface antigens; Cell surface determinants: for growth factors; for growth regulators

Family

Application16/668,717Parent of this continuation · filed 30 Oct 2019

US 2022/0396792 A1Pre-grant publication · published 15 Dec 2022

US 11,613,748 B2Parent of this continuation

US 2021/0130820 A1Published application

US 12,595,480 B2This patent

From the US filing history. Foreign family members aren’t listed.

Method for producing nucleic acid molecule, biomaterial, and method for producing biomaterial

Life of this patent

Method for producing nucleic acid molecule, biomaterial, and method for producing biomaterial

Life of this patent

Abstract

Claims9