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US 12,595,478 B2Granted
Crispr-Cas systems having destabilization domain
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, The Brigham and Women's Hospital, Inc.
InventorsAmit Choudhary, Basudeb Maji, Bernd Zetsche, Feng Zhang
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Abstract
The disclosure includes non-naturally occurring or engineered DNA- or RNA-guided nuclease systems, comprising guide-binding adaptors each associated with at least one destabilization domain (DD), along with compositions, systems and complexes involving such systems, nucleic acid molecules and vectors encoding the same, delivery systems involving the same, uses therefor.
Claims31
A light activatable stabilizing ligand of the formula:
- wherein R is selected from:
A transcriptional control system comprising;
- a fusion protein comprising:
- (i) one or more DHFR50 destabilization domains;
- (ii) a CRISPR-Cas system comprising a catalytically inactive Cas protein and a guide molecule, the guide molecule comprising a binding recognition site;
- (iii) an adaptor protein configured to bind to a binding recognition site of the guide molecule of the CRISPR-Cas system, and
- (iv) a transcriptional activation domain or a transcriptional repressor domain;
- and
- a light activatable stabilizing ligand of the formula:
- wherein R is selected from:
5 more elements
Dependencies are inferred from the claim text.