High throughput genetic barcoding and analysis methods
AssigneeWilliam Marsh Rice University
InventorsDavid Yu Zhang, Ping Song, Omid Veiseh, Siavash Parkhideh, Sudip Mukherjee, Maria Isabel Ruocco, Boram Kim, Yuxuan Cheng
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Abstract
Provided herein are high-throughput methods for genetic barcoding and analysis, e.g., for tagging each biomaterial capsule with a barcode cell. These barcode cells are derived from patient samples, and thus embody natural human genetic variation. Also provided are SNP panels that can be used as genetic barcodes to identify the identity of a cell.
Claims16
A method for identifying a cell encapsulation material following in vivo screening of cell encapsulation materials, the method comprising (a) preparing capsules containing barcode cells using various encapsulation materials, wherein the barcode cells comprise a plurality of SNPs that serve as a genetic barcode for each cell encapsulation material;
- (b) implanting the capsules into a test subject;
- (c) explanting the capsules after a set period of time;
- and (d) determining the sequence of the plurality of SNPs in the barcode cells of each explanted capsule, thereby identifying the cell encapsulation material of each capsule.
A method for identifying a cell encapsulation material following in vivo screening of cell encapsulation materials, the method comprising (a) preparing a plurality of barcode cells derived from one or more subjects, wherein each composition of barcode cells comprises a unique profile of a plurality of SNPs that serve as a genetic barcode for each cell encapsulation material;
- (b) fabricating capsules using various encapsulation materials and barcode cells;
- (c) implanting the capsules into a test subject;
- (d) explanting the capsules after a set period of time;
- and (d) determining the sequence of the plurality of SNPs in the barcode cells of each explanted capsule, thereby identifying the cell encapsulation material of each capsule.
Dependencies are inferred from the claim text.