Cell observation device, electrostimulation device, and cell observation method

A system for observing a cell comprising:

• a sample case including at least one holder;
• a dark box configured to accommodate the sample case including the at least one holder holding a sample including the cell, wherein the sample case having a bottom portion that is formed of a material which can transmit light from the sample;
• a light-guiding optical system including a lens, the light-guiding optical system disposed under the bottom portion of the sample case and configured to guide a two-dimensional image;
• a camera configured to capture the two-dimensional image that is guided by the light-guiding optical system and output movie data of the two-dimensional image;
• an electrical stimulator including an electrode pair having a first electrode and a second electrode, wherein the electrode pair is disposed in the holder;
• and
• a computer configured to set an analysis area on the two-dimensional image and analyze optical intensity in the analysis area,
• wherein the electrode pair has a structure in which a tip of the first electrode on a bottom side of the holder extends more than a tip of the second electrode on the bottom side of the holder, and
• wherein the electrode pair is positioned by bringing the tip of the second electrode into contact with a bottom face of the holder and by allowing a space between the tip of the first electrode and the bottom face of the holder, when the electrode pair is inserted into the holder.