Anti-CTLA4 monoclonal antibodies and chimeric antigen receptors
AssigneeNantBio, Inc.
InventorsClifford Anders Olson, Shiho Tanaka, Kayvan Niazi, Melanie Hermreck, Thomas H. King, Zhimin Guo
Life of this patent
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Abstract
Compositions and methods are presented in which selected polypeptide compounds bind to CTLA-4. Most typically, binding is mediated by selected VH and/or VL domains, and preferred compounds are prepared as scFv, IgG, or CAR.
Claims8
An antibody or fusion protein comprised thereof, comprising:
- a variable heavy chain (VH) segment and a variable light chain (VL) segment respectively consisting of SEQ ID NO:60 and SEQ ID NO:
- 63, SEQ ID NO:61 and SEQ ID NO:63, SEQ ID NO:
- 62 and SEQ ID NO:63, SEQ ID NO:64 and SEQ ID NO:67, SEQ ID NO:65 and SEQ ID NO:67, SEQ ID NO:66 and SEQ ID NO:67, SEQ ID NO:
- 68 and SEQ ID NO:69, or SEQ ID NO:70 and SEQ ID NO:71.
An antibody, comprising a heavy chain comprising a first amino acid sequence of SEQ ID NO. 68 and a light chain comprising a second amino acid sequence of SEQ ID NO. 69, or a heavy chain comprising a first amino acid sequence of SEQ ID NO. 70 and a light chain comprising a second amino acid sequence of SEQ ID NO. 71.
A recombinant nucleic acid, comprising:
- a first nucleic acid segment encoding a variable heavy chain (VH) peptide sequence and second nucleic acid segment encoding a variable light chain (VL) peptide sequence, wherein the VH and VL peptide sequences combine to form an antibody, scfv, or fusion proteins derived therefrom, and wherein the VH and VL peptides respectively consist of SEQ ID NO:60 and SEQ ID NO:63, SEQ ID NO:61 and SEQ ID NO:63, SEQ ID NO:62 and SEQ ID NO:63, SEQ ID NO:
- 64 and SEQ ID NO:67, SEQ ID NO:
- 65 and SEQ ID NO:67, SEQ ID NO:
- 66 and SEQ ID NO:67, SEQ ID NO:
- 68 and SEQ ID NO:69, or SEQ ID NO:
- 70 and SEQ ID NO:71;
- and wherein the first and second segments are optionally in a bicistronic arrangement.
A recombinant nucleic acid, comprising:
- a first nucleic acid segment encoding a first amino acid sequence of SEQ ID NO. 68 and second nucleic acid segment encoding a second amino acid sequence of SEQ ID NO. 69, or a first nucleic acid segment encoding a first amino acid sequence of SEQ ID NO. 70 and a second nucleic acid segment encoding a second amino acid sequence of SEQ ID NO. 71.
Dependencies are inferred from the claim text.