Method for synthesizing nucleotide strands

Abstract

The present disclosure discloses a method for synthesizing nucleotide strands, relating to the biology field. The synthesizing method includes regulating a temperature for synthesizing the nucleotide strands to ≥42° C. or performing a procedure of high-temperature denaturation-low-temperature annealing for at least one time during the synthesis, thus achieving efficient ligation of natural nucleic acid fragments and/non-natural nucleic acid fragments. This method can effectively reduce byproducts produced during the synthesis of nucleotide strands, improve the efficiency of synthesis of nucleotide strands of interest, and facilitate large-scale production of high-quality nucleotide strands.

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