Bispecific chimeric antigen receptor that binds CD19 and CD20, encoding nucleic acid molecules thereof and methods of use thereof to treat cancer

A population of immune effector cells, comprising a bispecific chimeric antigen receptor (CAR) molecule comprising:

• (a) first antigen binding domain that binds to CD20 comprising a heavy chain variable domain (VH) comprising an amino acid sequence of SEQ ID NO:
• 512, a linker comprising an amino acid sequence of SEQ ID NO:
• 541, and a light chain variable domain (VL) comprising an amino acid sequence of SEQ ID NO:
• 514;
• (b) a (Gly4-Ser)5 linker;
• (c) a second antigen binding domain that binds to CD19 comprising an amino acid sequence of SEQ ID NO:
• 771;
• (d) a CD8 hinge comprising an amino acid sequence of SEQ ID NO:
• 799;
• (e) a CD8 transmembrane domain comprising an amino acid sequence of SEQ ID NO:
• 801;
• (f) a 4-1BB costimulatory domain comprising an amino acid sequence of SEQ ID NO:
• 803;
• and
• (g) a CD3z intracellular signaling domain comprising an amino acid sequence of SEQ ID NO:
• 807.