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US 12,594,308 B2Granted
Methods of preparing a postbiotic composition
AssigneeScience Power Srl
InventorFederica Nigro
Life of this patent
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Abstract
The disclosure provides methods of preparing a postbiotic composition comprising multiple (i.e., two or more) separate microbial fermentations, which may be performed in parallel or sequential steps.
Claims15
A method of preparing a postbiotic composition comprising a sequential fermentation process, said sequential fermentation process comprising the steps of:
- (i) inoculating a culture medium with a first fermenting microorganism;
- (ii) fermenting the culture medium under conditions suitable for fermentation by the first fermenting microorganism, thereby obtaining a first fermentation matrix comprising the first fermenting microorganism;
- (iii) inactivating the first fermenting microorganism in the first fermentation matrix to obtain a first fermentation product comprising the inactivated first fermenting microorganism and the first fermentation matrix;
- (iv) inoculating the first fermentation product of step (iii) with a second fermenting microorganism;
- (v) fermenting the first fermentation product under conditions suitable for fermentation by the second fermenting microorganism, thereby obtaining a second fermentation product comprising the second fermenting microorganism and the inactivated first fermenting microorganism;
- (vi) inactivating the second fermenting microorganism in the second fermentation product to obtain a postbiotic composition comprising the inactivated first fermenting microorganism and the inactivated second fermenting microorganism, wherein at least one of the fermenting microorganisms is Lacticaseibacillus paracasei NPBO1;
- and
- optionally, after inactivating the second fermenting microorganism of step (vi), repeating steps (iv) to (vi) one or more times with an additional fermenting microorganism.
5 more elements
A method of preparing a postbiotic composition comprising a sequential fermentation process, said sequential fermentation process comprising the steps of:
- (i) inoculating a culture medium with a first fermenting microorganism;
- (ii) fermenting the culture medium under conditions suitable for fermentation by the first fermenting microorganism, thereby obtaining a first fermentation matrix comprising the first fermenting microorganism;
- (iii) inactivating the first fermenting microorganism in the first fermentation matrix to obtain a first fermentation product comprising the whole of the inactivated microbial biomass of the first fermenting microorganism and the first fermentation matrix;
- (iv) inoculating the first fermentation product of step (iii) with a second fermenting microorganism;
- (v) fermenting the first fermentation product under conditions suitable for fermentation by the second fermenting microorganism, thereby obtaining a second fermentation product comprising the second fermenting microorganism and the whole of the inactivated microbial biomass of the first fermenting microorganism;
- (vi) inactivating the second fermenting microorganism in the second fermentation product to obtain a postbiotic composition comprising the whole of the inactivated microbial biomass of the first fermenting microorganism and the whole of the inactivated microbial biomass of the second fermenting microorganism, wherein at least one of the fermenting microorganisms is Lacticaseibacillus paracasei NPBO1;
- and
- optionally, after inactivating the second fermenting microorganism of step (vi), repeating steps (iv) to (vi) one or more times with an additional fermenting microorganism.
5 more elements
Dependencies are inferred from the claim text.