Potentiation of antibiotic effect
AssigneeThe University of North Carolina at Chapel Hill
InventorsBrian Conlon, Sarah Conlon, Lauren Radlinski
Life of this patent
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Abstract
The present invention relates to compositions, kits and methods using combinations of surfactants or cell wall-hydrolyzing enzymes with antibiotics to potentiate the antibiotic effect against bacterial infections. In some embodiments, the surfactant is a pore-forming biosurfactant such as rhamnolipids and the antibiotic is an aminoglycoside antibiotic such as tobramycin.
Claims18
A method of treating a bacterial infection comprising a biofilm in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a surfactant and at least one of an aminoglycoside antibiotic or vancomycin, wherein:
- (a) the surfactant consists of palmitoleic acid;
- (b) the aminoglycoside antibiotic comprises streptomycin, kanamycin A, amikacin, dibekacin, sisomicin, netilmicin, neomycin B, neomycin C, neomycin E, or any combination thereof;
- (c) the surfactant synergizes with the aminoglycoside antibiotic or vancomycin to decrease the minimum inhibitory concentration (MIC) of the aminoglycoside antibiotic or vancomycin;
- wherein the bacterial infection comprising a biofilm is caused by gram-positive bacteria;
- and
- wherein the bacterial infection comprising a biofilm is treated.
A method of reducing the risk of recurrence of a bacterial infection in a subject in need thereof, comprising administering to the subject having a bacterial infection comprising a biofilm a therapeutically effective amount of a surfactant and at least one of an aminoglycoside antibiotic or vancomycin, wherein:
- (a) the surfactant consists of palmitoleic acid;
- (b) the aminoglycoside antibiotic comprises streptomycin, kanamycin A, amikacin, dibekacin, sisomicin, netilmicin, neomycin B, neomycin C, neomycin E, or any combination thereof;
- (c) the surfactant synergizes with the aminoglycoside antibiotic or vancomycin to decrease the MIC of the aminoglycoside antibiotic or vancomycin;
- wherein the bacterial infection comprising a biofilm is caused by a gram-positive bacteria;
- and
- wherein the risk of recurrence of a bacterial infection is reduced.
A method of disrupting a biofilm, comprising contacting the biofilm with an effective amount of a surfactant and at least one of an aminoglycoside antibiotic or vancomycin, wherein:
- (a) the surfactant consists of palmitoleic acid;
- (b) the aminoglycoside antibiotic comprises streptomycin, kanamycin A, amikacin, dibekacin, sisomicin, netilmicin, neomycin B, neomycin C, neomycin E, or any combination thereof;
- (c) the surfactant synergizes with the aminoglycoside antibiotic or vancomycin to decrease the MIC of the aminoglycoside antibiotic or vancomycin;
- wherein the biofilm is from a gram-positive bacteria;
- and
- wherein the biofilm is disrupted.
Dependencies are inferred from the claim text.