Artificial vitreous humor for the investigation of drugs and drug formulations

A method for analyzing the behavior of an artificial humor composition comprising the steps in order:

• (i) providing an artificial vitreous humor composition in an in vitro environment;
• (ii) applying a substance to the artificial vitreous humor;
• and
• (iii) determining at least one property of the artificial humor composition,
• wherein the artificial vitreous humor composition comprises,
• (A) a phosphate buffer, wherein the phosphate buffer is phosphate buffer saline (PBS) in the range from 0.001 to 0.2 M and has a pH value in the range from 7.0 to 7.7,
• (B) 64.6 μM creatinine, 2.2 mM glucose, 7.6 mM urea, 580 μM xanthine, 309 μM hypoxanthine, 10.5 mM sodium lactate and 200 μM glutathione, and
• (C) 40 mg/L type II collagen and 0.6% w/v sodium hyaluronate, and
• wherein the applied substance is left in the artificial vitreous humor composition for up to 360 days, prior to step (iii) and the artificial vitreous humor composition remains at a pH having a value in the range from 7.0 to 7.7.