Back to results
US 12,576,189 B2Granted
Nerve grafts containing regenerative compounds, methods of making the same, and methods of treatment using the same
AssigneeAxogen Corporation
InventorsKasra Tajdaran, Angelo Scopelianos
Life of this patent
Estimating the term…
Abstract
A method of preparing a nerve graft includes submerging a nerve graft in a solution including FK506 and a solvent to promote incorporation of FK506 into the nerve graft. A tissue graft includes nerve tissue and FK506 incorporated within the nerve tissue. In the tissue graft, the FK506 is free of hydrogel and not encapsulated.
Claims20
A method of preparing a nerve graft, the method comprising:
- submerging a nerve graft comprising acellular nerve tissue with hydrophobic portions in a solution comprising FK506 and a solvent that comprises between about 1% and about 15% by volume dimethyl sulfoxide (DMSO) to promote incorporation of FK506 into the nerve graft, the solution being free of polymer.
A method of preparing a nerve graft, the method comprising:
- submerging a nerve graft comprising acellular nerve tissue with hydrophobic portions in a solution comprising FK506 and a solvent to promote incorporation of FK506 into the nerve graft, the solution being free of polymer;
- and sterilizing the nerve graft by exposing the nerve graft to gamma irradiation.
A method of preparing a nerve graft, the method comprising:
- exposing a nerve graft comprising acellular nerve tissue with hydrophobic portions to a solution comprising FK506, while the nerve graft is within a container, such that the FK506 is at least partially incorporated within one or more of the hydrophobic portions of the nerve graft such that the FK506 is present in greater concentrations within the one or more hydrophobic portions as compared to a non-hydrophobic portion of the acellular nerve tissue;
- sterilizing the nerve graft while the nerve graft and the solution are within the container;
- and
- storing the nerve graft and the solution in the container.
1 more element
A method of preparing a nerve graft, the method comprising:
- submerging a nerve graft that includes acellular nerve tissue with hydrophobic portions in a solution, the solution comprising FK506 and DMSO and being free of polymer, to promote incorporation of FK506 throughout the nerve tissue of the nerve graft such that the FK506 is present in greater concentrations within the hydrophobic portions as compared to a non-hydrophobic portion of the acellular nerve tissue.
Dependencies are inferred from the claim text.