Isolated nucleic acid molecule and application thereof

An isolated nucleic acid molecule, comprising, in a 5′-to-3′ direction, a liver-specific expression regulatory element and a gene of interest operably linked to the liver-specific expression regulatory element,

• wherein the liver-specific expression regulatory element comprises, in order in the 5′-to-3′ direction:
• a promoter derived from the dog serpinAl gene or functional fragment thereof, a promoter derived from the xenopus laevis vitellogenin A2 gene or functional fragment thereof, a promoter derived from the xenopus laevis albumin gene or the functional fragment thereof, and a promoter derived from the human serpinAl gene or functional fragment thereof, wherein, the promoter derived from the dog serpinAl gene or functional fragment thereof comprises the nucleotide sequence of SEQ ID NO:
• 2;
• the promoter derived from the xenopus laevis vitellogenin A2 gene or functional fragment thereof comprises the nucleotide sequence of SEQ ID NO:
• 3;
• the promoter derived from the xenopus laevis albumin gene or functional fragment thereof comprises the nucleotide sequence of SEQ ID NO:
• 4;
• and
• the promoter derived from the human serpinAl gene or functional fragment thereof comprises the nucleotide sequence of SEQ ID NO:
• 5.