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US 12,523,575 B2Granted
Method and apparatus for collecting bacteria
AssigneeHitachi High-Tech Corporation
InventorsKiyotaka Sugiyama, Hiroko Fujita, Tsuyoshi Sonehara
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Abstract
The present invention relates to a method, a kit and an apparatus for pretreatment of bacterial test. More specifically, the present invention relates to a method, a kit, and an apparatus for continuously performing separation of blood cells and bacteria from a sample containing impurities such as blood cells, destruction of blood cells, and collection of bacteria.
Claims11
A method for collecting microorganisms, the method comprising:
- a step of introducing a sample into a first container having at least one through hole in a side thereof;
- a step of having the through hole of the first container to be enclosed within and connected to a second container;
- a first separation step of performing centrifugation on the first container and the second container with a first centrifugal force to separate the sample into a solution containing microorganisms and a solution containing blood cells;
- and
- a second separation step of discharging the solution containing microorganisms from the through hole to the second container to collect the solution containing microorganisms in the second container.
2 more elements
A microorganism collecting apparatus comprising:
- a centrifuge configured to perform centrifugation on a first container and a second container together;
- and
- a control unit configured to control centrifugal force of the centrifuge,
- wherein
- the first container comprises at least one through hole in a side thereof, and a sample is to be introduced into the first container,
- the second container is configured to enclose and connect to the through hole of the first container, and
- the control unit is configured to control the centrifugal force of the centrifuge to perform:
- a first separation using a first centrifugal force that separates the sample introduced into the first container into a solution containing microorganisms and a solution containing blood cells;
- and
- a second separation using a second centrifugal force greater than the first centrifugal force, the second separation causing the solution containing microorganisms to be discharged from the through hole of the first container to the second container following the first separation.
7 more elements
Dependencies are inferred from the claim text.